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Rabbit Anti-GPLD1 Polyclonal Antibody#abs139270

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Description

Catalog-specification	Delivery time	USD price
abs139270-100ug	1-2 Weeks	301.0
abs139270-50ug	1-2 Weeks	201.0

Overview
catalog	abs139270
Other names	Glycoprotein phospholipase D, also known as GPI-PLD or GPI-specific phospholipase D, is an enzyme that specifically cleaves glycosylphosphatidylinositol (GPI) anchor from the protein in mammalian cells. It is encoded by the GPLD1 gene and is also referred to as phosphatidylinositol-glycan-specific phospholipase D or PI-GPLD. The enzyme is involved in various cellular processes such as signal transduction and membrane trafficking by cleaving the GPI anchor from proteins, releasing them into the extracellular space. The enzyme has also been implicated in the regulation of the complement system and immune response. The glycosyl-phosphatidylinositol-specific phospholipase D has gained attention as a potential therapeutic target for various diseases, including cancer and diabetes. Overall, the study of GPI-PLD has provided valuable insights into the complex and diverse roles played by lipids in cellular physiology.
Source	Rabbit
Specificity	The GPLD1 Antibody is capable of detecting total GPLD1 levels present in the body. This detection is based on the level of endogenous GPLD1 in the body.
Species Reactivity	Human;Mouse
Antigen	GPLD1
Application	For Western blot, dilutions of 1:1000 to 1:3000 are recommended, while for immunohistochemistry, a dilution of 1:200 is recommended. In ELISA using peptides, dilutions of 1:20000 to 1:40000 are suggested. It is important to follow proper dilution recommendations to ensure accurate and reliable results in these assays. Dilution factors can vary depending on the specific antibody or peptide used, so it is important to check product instructions carefully. Proper sample preparation and handling is also essential in achieving optimal results.
Immunogen	A synthesized peptide derived from human GPLD1.
MW	92 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Ensure that the storage temperature remains at -20 °C for a period of one year in order to maintain the product's quality. It is important to prevent subjecting the product to multiple cycles of freezing and thawing.
Storage buffer	The Rabbit IgG solution is formulated in a pH 7.4 phosphate buffered saline, containing 150mM NaCl and 0.02% sodium azide. This solution also contains 50% glycerol to ensure stability during storage at -20 °C. The solution can be safely stored for up to 12 months from the date of receipt, ensuring consistent and reliable results when used in research or diagnostic applications.
Target
Background	This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.
Celluar localization	Extracellular region or secreted;Lysosome;
UniPort	P80108