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Rabbit Anti-INPPL1 Polyclonal Antibody#abs139267

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Description

Catalog-specification	Delivery time	USD price
abs139267-100ug	1-2 Weeks	301.0
abs139267-50ug	1-2 Weeks	201.0

Overview
catalog	abs139267
Other names	The protein known as 4; 5-trisphosphate 5-phosphatase 2 or SH2 domain-containing inositol 5' phosphatase 2 is also referred to by several other names, including inositol polyphosphate phosphatase like 1, OPSMD, INPPL-1, and SHIP2. This enzyme plays a key role in the regulation of cellular signaling pathways by specifically dephosphorylating phosphatidylinositol 3,4,5 trisphosphate (PIP3) to form phosphatidylinositol 3,4-bisphosphate (PIP2). SHIP2 contains an SH2 domain which allows it to interact with other signaling proteins and regulate their activity. Dysfunction of SHIP2 has been implicated in various diseases including diabetes, cancer, and metabolic disorders. Overall, this protein is an important player in the complex network of cellular signaling pathways and represents a promising target for therapeutic intervention.
Source	Rabbit
Specificity	Total endogenous levels of INPPL1 can be detected using the INPPL1 Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	INPPL1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human INPPL1.
MW	139kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum. The resulting purified antiserum was of high quality and similarity to the original.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. This can ensure that the product retains its efficacy and remains useful for an extended period of time. So, always keep in mind to follow the storage guidelines to make the most of the product.
Storage buffer	This product is Rabbit IgG that has been suspended in a solution consisting of phosphate buffered saline with pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. For optimal preservation, it should be stored at a temperature of -20°C. You can expect it to remain stable for a period of 12 months from the date of receipt.
Target
Background	Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling (PubMed:21624956). Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification.
Tissue specificity	Widely expressed, most prominently in skeletal muscle, heart and brain. Present in platelets. Expressed in transformed myeloid cells and in primary macrophages, but not in peripheral blood monocytes.
Posttranslational modification	Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as insulin, growth factors such as EGF or PDGF, chemokines, integrin ligands and hypertonic and oxidative stress. May be phosphorylated upon IgG receptor FCGR2B-binding. Phosphorylated at Tyr-986 following cell attachment and spreading. Phosphorylated at Tyr-1162 following EGF signaling pathway stimulation. Phosphorylated at Thr-958 in response to PDGF.
Celluar localization	Cytoskeleton;Cytosol;Golgi apparatus;Plasma Membrane;
UniPort	O15357