Phospho-AMPK Alpha (Thr172) Rabbit Polyclonal Antibody#abs131048

abs131048-50ug

1-2 Weeks

201

abs131048-100ug

1-2 Weeks

301

Other names

Source

Rabbit

Specificity

The Phospho-AMPK alpha (Thr172) Antibody is designed to specifically recognize and bind to AMPK alpha proteins that are phosphorylated at Threonine 172. This antibody enables the detection of endogenous levels of AMPK alpha only when it is phosphorylated at this specific site. By targeting this phosphorylation event, the antibody provides valuable insights into the activation status of AMPK alpha.

Predictive reaction species

Zebrafish;Chicken;Rabbit;Xenopus;Dog;Bovine;Sheep;Pig;

Application

The recommended dilutions for using this antibody are as follows: for Western blotting, use 1:500-1:2000; for immunohistochemistry, use 1:50-1:200; for immunofluorescence, use 1:100-500; and for ELISA (peptide), use 1:20000-1:40000. It is important to note that these dilutions are based on the original text information and should be adjusted accordingly for optimal results. Follow the manufacturer's instructions and consult the literature or a qualified expert to determine the appropriate dilution for your specific application. By using this antibody at the recommended dilutions, you can ensure high-quality and reliable results in your experiments.

The phosphorylation site of Threonine 172 is utilized to develop a synthesized peptide obtained from human AMPK alpha. It is essential to maintain a high degree of similarity while rearranging the content to ensure it remains consistent with the original text. However, please note that dialogue in a completely different manner, as generated by the language model, is not suitable for this task.

Properties

1mg/ml

Purification

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Clonality

Polyclonal Antibody

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

Q13131/P54646