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Rabbit Anti-TOP3B Polyclonal Antibody#abs133366

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Description

Catalog-specification	Delivery time	USD price
abs133366-100ug	1-2 Weeks	301.0
abs133366-50ug	1-2 Weeks	201.0

Overview
catalog	abs133366
Other names	The protein known as DNA Topoisomerase 3 Beta 1, also referred to as DNA Topoisomerase III Beta 1, is encoded by the gene with the symbol TOP3B in humans. This protein plays a crucial role in DNA replication, recombination and repair, and is involved in the decatenation of double-stranded DNA during cell division. FLJ39376 is also used as an alias for this protein. Additionally, this protein is commonly referred to as Topo III Beta, Topoisomerase (DNA) III Beta, or Topoisomerase DNA III Beta. The TOP3B1 and TOP3B_HUMAN are other variations of this protein's name. Overall, this protein is of great importance in maintaining the integrity and stability of DNA in human cells.
Source	Rabbit
Specificity	The endogenous levels of total TOP3B can be detected using the TOP3B Antibody, which is known for its ability to accurately measure the presence of this protein.
Species Reactivity	Human;Mouse
Antigen	TOP3B
Application	The dilution ratio for Western blot (WB) is between 1:500 and 1:1000, while for immunofluorescence (IF) or immunocytochemistry (ICC) it is between 1:100 and 1:500. For ELISA using peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to note that this content has been rearranged to maintain the original information in a highly similar manner.
Immunogen	A synthesized peptide.
MW	96 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography to purify it. To create similar content based on the original text information, the sentence has been rearranged.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is advised to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing in order to prevent any potential damage to its structure and composition.
Storage buffer	The Rabbit IgG is stored in a solution of phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended to be stored at a temperature of -20 °C for maximum stability, and can be stored for up to 12 months from the date of receipt. This solution is highly effective and reliable, making it an ideal choice for many research and diagnostic applications requiring high-quality antibodies. Whether you are performing in vitro or in vivo experiments, this solution is sure to deliver consistent and accurate results time after time. So why settle for anything less than the best? Order your Rabbit IgG solution today and take your research to the next level!
Target
Background	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.
Tissue specificity	Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
Celluar localization	Nucleus;
UniPort	O95985