Phospho-IRF-3 (Ser386) Antibody#abs140593
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Description
| Catalog-specification | Delivery time | USD price |
abs140593-50ug | In Stock | 201.0 |
abs140593-100ug | In Stock | 301.0 |
Please note that the price provided above is only for your reference. If you require a detailed breakdown of the price, kindly contact our seller Vecent who will be happy to assist you. It's important to clarify that any further information you receive is based on the original text provided to avoid any misinterpretation. Thank you for your understanding.
| Overview | |
catalog | abs140593 |
Description | Interferon regulatory factor 3, also known as IRF3, belongs to the family of interferon regulatory transcription factors (IRFs). Within the cytoplasm, IRF3 is predominantly found in an inactive state. However, when specific serine/threonine phosphorylation occurs, it undergoes a transformation and forms a complex with CREBBP. |
| Other names | IRF-3, also known as Interferon Regulatory Factor 3, is a protein encoded by the IIAE7 gene in humans. It plays a significant role in the immune system and is involved in the regulation of interferon production. IRF-3 is commonly referred to as IRF3 or IRF3_HUMAN, and it has also been designated as MGC94729 in some studies. This protein is essential for the activation of type I interferon genes in response to viral infection or other stimuli. Rearranging the given content, we can say that IRF-3, also known as Interferon Regulatory Factor 3, is a crucial protein encoded by the IIAE7 gene in humans. It regulates the production of interferon and plays a significant role in the immune system. IRF-3 is commonly referred to as IRF3 or IRF3_HUMAN and has been designated as MGC94729 in some studies. Its activation is vital for the expression of type I interferon genes when the body is infected by viruses or exposed to other stimuli. |
| Source | Rabbit |
| Specificity | The antibody for Phospho-IRF-3 (Ser386) specifically recognizes and detects the presence of phosphorylated IRF-3 at Serine 386 in endogenous samples. By targeting this specific phosphorylation site, the antibody allows researchers to study the levels and localization of active IRF-3 within cells. |
| Species Reactivity | Human;Rat |
| Antigen | IRF-3 |
| Application | The recommended dilution for Western blot is 1:500-1:2000, for immunohistochemistry is 1:50-1:200, for immunofluorescence/immunocytochemistry is 1:100-1:500, and for ELISA with peptide is 1:20000-1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results in your experiments. Diluting the primary antibody within these specified ranges will help optimize the signal-to-noise ratio and ensure proper detection of the target of interest. |
| Immunogen | A peptide synthesized from a region of human IRF-3 containing Ser386, the site of phosphorylation, is available. It is composed of amino acids that closely resemble those found in the original sequence. |
| Properties | |
Concentration | 1mg/ml |
| purification | Through sequential chromatography on phospho- and non-phospho-peptide affinity columns, we were able to obtain a purified rabbit serum antibody by affinity purification. The resulting antibody is highly specific and similar in composition to the original serum, allowing for effective detection and targeting of specific proteins or molecules. This process ensures the quality and purity of the final product, making it a valuable tool in various applications such as biochemistry and biomedical research. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Ensure that the product is stored at -20 °C for a duration of one year in order to maintain its quality. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can cause adverse effects. |
| Storage buffer | 150mM NaCl、0.02%50%(pH 7.4)IgG。，。 |
Target | |
Background | Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages. |
| Tissue specificity | Expressed constitutively in a variety of tissues. |
| Posttranslational modification | Constitutively phosphorylated on many Ser/Thr residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Phosphorylated at Ser-396 by IKBKE upon ssRNA viral infection. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. Phosphorylation at Ser-386 by TBK1 results in oligomerization. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection.(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. |
| Celluar localization | Nucleus |
| UniPort | Q14653 |
Western blot analysis of IRF-3 phosphorylation expression in Insulin treated HT29 whole cell lysates,The lane on the left was treated with the antigen-specific peptide.
This product is for research use only, not for use in diagnostic prodecures or in human.
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