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Rabbit Anti-AS250 Polyclonal Antibody#abs140597

Please note that the price mentioned is only for your reference. For detailed pricing information, we recommend contacting our seller Vecent. Thank you. Western blot analysis of extracts from Jurkat cells, using AS250 antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs140597-100ug	1-2 Weeks	301.0
abs140597-50ug	1-2 Weeks	201.0

Please note that the price mentioned is only for your reference. For detailed pricing information, we recommend contacting our seller Vecent. Thank you.

Overview
catalog	abs140597
Other names	Akt substrate AS250, also known as AS250 or p220, is a 250 kDa substrate of the Akt protein. It is encoded by various gene names such as bA287B20.1, dJ1049G11, dJ1049G11.4, KIAA1272, Ral GTPase-activating protein alpha subunit 2, Ral GTPase-activating protein subunit alpha-2, RALGAPA2, RapGAPalpha2, RGPA2_HUMAN, and RP11-470C13.2.
Source	Rabbit
Specificity	The endogenous levels of total AS250 can be detected using the AS250 Antibody. A highly similar content can be generated by rearranging the original text information.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Zebrafish;Rabbit;Pig;Dog;Horse;Bovine;Sheep;
Antigen	AS250
Application	For western blotting, the recommended dilution range is 1:500 to 1:1000. When it comes to immunohistochemistry, a dilution range of 1:50 to 1:200 is suggested. For immunofluorescence/immunocytochemistry, a dilution range of 1:100 to 1:500 should be used. Lastly, when performing an ELISA assay using a peptide, the recommended dilution range is 1:20000 to 1:40000. These recommended dilutions should be taken into consideration when using the specified assays in order to obtain optimal results.
Immunogen	A synthesized peptide.
MW	210 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent peptide affinity chromatography purification. An effective method for isolating and purifying target proteins or antibodies, this process is crucial for ensuring the final product's quality.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the integrity and quality of our product, it is recommended that you store it at a temperature of -20 °C for a period of one year. It is essential to avoid exposing the product to repeated freeze/thaw cycles which can cause degradation and damage over time. Keeping the product at a consistent and stable temperature is crucial for maintaining its efficacy and performance. With proper storage, you can ensure the longevity and optimal use of our product.
Storage buffer	The Rabbit IgG comes in a solution of phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide and is mixed with 50% glycerol. It is recommended to store the solution at a temperature of -20 °C in order to maintain its integrity. This solution can remain stable for up to 12 months from the day it was received, which makes it a reliable product for long-term usage.
Target
Background	Catalytic subunit of the heterodimeric RalGAP2 complex which acts as a GTPase activator for the Ras-like small GTPases RALA and RALB.
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q2PPJ7