Phospho-COT (Thr290) Rabbit Polyclonal Antibody#abs130923

abs130923-50ug

1-2 Weeks

201

abs130923-100ug

1-2 Weeks

301

Description

Cells with oncogenic transforming activity identified this gene, which encodes a member of the serine/threonine protein kinase family. Being capable of activating both the MAP kinase and JNK kinase pathways, this kinase demonstrates its functional versatility. By rearranging the original content, a highly similar context can be created.

Other names

The AURA2 gene, also known as COT or Cancer Osaka thyroid oncogene, plays a crucial role in the development and progression of various types of cancer. This gene encodes a serine/threonine protein kinase known as MEKK8, or mitogen-activated protein kinase kinase kinase 8, TPL-2, or Tumor progression locus 2.
The proto-oncogene c-Cot is one form of this gene, and it is involved in the transformation of Ewing sarcoma, a rare form of bone cancer. Additionally, the ESTF or Ewing sarcoma transformant, FLJ10486, and MAP3K8 are also involved in regulating cell growth and division and are highly expressed in certain types of cancer.
The COT proto-oncogene serine/threonine protein kinase is another form of this gene that plays a role in the regulation of cell growth and differentiation, and is thought to be involved in tumorigenesis. Therefore, understanding the function of the AURA2 gene and its various forms is crucial for developing new therapeutic strategies to treat cancer.

Source

Rabbit

Specificity

The Phospho-COT (Thr290) Antibody is specifically designed to target and detect the levels of phosphorylated COT at threonine 290. This antibody is highly sensitive and can accurately detect endogenous levels of COT phosphorylation only when it occurs at this specific residue. It is a valuable tool for researchers studying COT signaling pathways and the role of COT phosphorylation in disease development and progression. By using this antibody, scientists can gain a better understanding of the cellular mechanisms that regulate COT activity and how these processes contribute to various physiological and pathological processes.

Predictive reaction species

Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;

Application

The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, a dilution range of 1:500 to 1:2000 is recommended, while for immunohistochemistry, a range of 1:50 to 1:200 is suggested. For immunofluorescence or immunocytochemistry, a dilution range of 1:100 to 1:500 should be used. When using the antibody in ELISA assays with a peptide substrate, a dilution range of 1:20000 to 1:40000 can be used. It is important to note that these dilution ranges are recommendations and may need to be adjusted based on the specific experimental conditions and the quality of the antibody used.

One possible rearrangement:
A peptide that mimics human COT and includes the phosphorylation site at Threonine 290 has been synthesized. The sequence of this peptide closely resembles the original one in order to ensure a high level of similarity. This peptide may be used in various research applications to study the function and regulation of COT in normal and diseased states. Its design and production required careful consideration of the structural and chemical properties of the native protein, as well as the techniques of peptide synthesis and purification. Overall, this peptide represents a valuable tool for investigating the biology of COT and its role in cellular signaling pathways.

Properties

1mg/ml

Purification

The purified rabbit serum was used to produce the antibody through a series of chromatography techniques on both phospho- and non-phospho-peptide affinity columns. The resulting product is highly specific and has great affinity for its target antigen. These techniques ensure that the antibody is pure and free from any other contaminants or impurities. The sequence of chromatography steps ensures that the antibody is specifically targeting the desired antigen, making it incredibly useful in research and diagnostic applications.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, keep the item at a temperature of -20 °C for a period of 12 months. It is important to avoid subjecting the item to repeated freeze and thaw cycles, as this can lead to degradation of the product.

Storage buffer

This product is a rabbit IgG solution that has been prepared in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl. The solution also contains 0.02% sodium azide and 50% glycerol for stabilization. It is recommended to store this product at -20 °C for optimal stability, with a shelf life of 12 months from the date of receipt.

Target

Background

 Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.

Tissue specificity

Expressed in several normal tissues and human tumor-derived cell lines.

Celluar localization

Cytosol;

P41279