Phospho-HNF4 Alpha (Ser313) Rabbit Polyclonal Antibody#abs130922

abs130922-50ug

1-2 Weeks

201

abs130922-100ug

1-2 Weeks

301

Description

This gene encodes a nuclear transcription factor that acts as a homodimer to bind DNA. As a result, it has the ability to regulate the expression of various genes, including hepatocyte nuclear factor 1 alpha. This particular transcription factor plays a crucial role in controlling the expression of numerous hepatic genes. By maintaining the original information, this rearrangement creates a highly similar content.

Other names

The gene FLJ39654 is also known as FRTS4 and encodes for the protein Hepatic nuclear factor 4 alpha, alternatively referred to as Hepatocyte nuclear factor 4, HNF-4-alpha, or simply HNF 4. This protein plays a critical role in regulating gene expression and is involved in the development and function of the liver, pancreas, and intestine.
Hepatocyte nuclear factor 4-alpha is a part of the nuclear receptor subfamily 2 group A member 1 and is responsible for binding to DNA and controlling gene transcription. Mutations in this gene have been associated with the genetic disorder MODY 1, which is characterized by early onset of type 2 diabetes.
In addition to its primary function in regulating gene expression, Hepatocyte nuclear factor 4-alpha has been shown to be involved in lipid metabolism, glucose homeostasis, and inflammation. Its importance in these physiological processes makes it a potential therapeutic target for metabolic disorders.
Overall, Hepatic nuclear factor 4 alpha, or HNF 4, is a critical regulator of gene expression and is involved in several important physiological processes. Understanding its function and regulation may lead to the development of new treatments for metabolic disorders such as type 2 diabetes.

Source

Rabbit

Specificity

The Phospho-HNF4 alpha (Ser313) Antibody is specifically designed to detect HNF4 alpha levels, but only when it is phosphorylated at Serine 313. This antibody is capable of detecting endogenous levels of HNF4 alpha, making it a valuable tool for any researcher working in the field of cellular signaling. By detecting this specific phosphorylation event, scientists can gain insights into the complex regulatory mechanisms that govern molecular signaling pathways. With the help of this antibody, researchers can better understand how cells respond to various environmental stimuli and how they maintain normal physiological functions.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Bovine;Horse;

Application

ELISA (peptide) 1:20000-1:40000, WB 1:500-1:2000, IHC 1:50-1:200, and IF/ICC 1:100-1:500 are the recommended dilution ranges for different laboratory techniques. These dilution ranges indicate the optimal concentration of the primary antibody to be used for each technique. For ELISA with peptides, a dilution range of 1:20000-1:40000 is suggested, while for Western Blotting (WB), a dilution range of 1:500-1:2000 is recommended. In Immunohistochemistry (IHC), the primary antibody should be diluted within the range of 1:50-1:200, and for Immunofluorescence/Immunocytochemistry (IF/ICC), a dilution range of 1:100-1:500 is advised. It is important to follow these dilution guidelines to achieve accurate and reliable results in the respective experimental techniques.

A peptide synthesized from the phosphorylation site of Serine 313 in human HNF4 alpha can be created. This synthetic peptide will bear a close resemblance to the original content in terms of the information provided.

Properties

1mg/ml

Purification

The affinity-purified antibody was obtained from rabbit serum, using a process that involved sequential chromatography on phospho- and non-phospho-peptide affinity columns. The serum was first subjected to affinity purification using the phospho-peptide column, and then the resulting purified sample was further processed on the non-phospho-peptide column. This method ensured a high degree of purity and specificity for the final antibody product.

Clonality

Polyclonal Antibody

To maintain optimum storage conditions, it is advised to store the product at -20 °C for a duration of one year. It is crucial to avoid subjecting the product to repetitive freeze and thaw cycles.

Storage buffer

This Rabbit IgG is suspended in phosphate buffered saline with a pH level of 7.4, along with 150mM of NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to be stored at -20°C and is deemed stable for up to 12 months from the date of receipt.

Target

Background

 Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine.

Celluar localization

Nucleus;

P41235