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Rabbit Anti-FTO Polyclonal Antibody#abs137898

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Description

Catalog-specification	Delivery time	USD price
abs137898-100ug	1-2 Weeks	301.0
abs137898-50ug	1-2 Weeks	201.0

Overview
catalog	abs137898
Other names	AlkB homolog 9; ALKBH9; Alpha-ketoglutarate-dependent dioxygenase FTO; AW743446; Fat mass and obesity-associated protein; FATSO, MOUSE, HOMOLOG OF; Fto; FTO_HUMAN; GDFD; KIAA1752; mKIAA1752; Protein fatso;
Source	Rabbit
Specificity	The FTO Antibody is specifically designed to recognize and measure the levels of total FTO protein present in the body. It is able to detect the endogenous expression of FTO, providing accurate information about its overall amount. By targeting and detecting this protein, the FTO Antibody allows for comprehensive analysis and research on its roles and potential significance in various biological processes.
Species Reactivity	Human
Predictive reaction species	Zebrafish;Rabbit;
Antigen	FTO
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human FTO.
MW	58 kDa
Properties
Concentration	1mg/ml
purification	We employed SulfoLink™ Coupling Resin in peptide affinity chromatography to successfully purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20°C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing as this could compromise its integrity. Therefore, it is advised to handle the product with care during storage and transportation to prevent any fluctuations in temperature. By following these guidelines, you can ensure that your product remains in optimal condition and can be used effectively when needed.
Storage buffer	Storage conditions for Rabbit IgG include storing it at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is advised to store the IgG in this manner for a period of 12 months from the date of receipt.
Target
Background	Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine (PubMed:18775698, PubMed:20376003). Specifically demethylates N6-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes (PubMed:22002720, PubMed:26458103). Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation (PubMed:18775698, PubMed:20376003). In particular, it is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells (PubMed:26287746).
Tissue specificity	Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
Celluar localization	Nucleus;
UniPort	Q9C0B1