Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-Histone H4K20me1 Polyclonal Antibody#abs136468

Notice: Please note that the price provided is for reference purposes only. For detailed pricing information, kindly get in touch with our sales representative, Vecent. We recommend reaching out to Vecent for more accurate and specific details regarding pricing. Western blot analysis of extracts...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136468-100ug	1-2 Weeks	301.0
abs136468-50ug	1-2 Weeks	201.0

Overview
catalog	abs136468
Description	In eukaryotes, histones play a critical role in the formation of the chromosomal fiber's nucleosome structure. This intricate structure comprises approximately 146 base pairs of DNA wrapped around an octamer of four core histones - H2A, H2B, H3, and H4. The compacting of the chromatin fiber occurs via a linker histone, H1, interacting with the DNA between nucleosomes and creating higher-order chromatin structures. The histone H4 family contains this gene, which is assigned to a histone cluster on chromosome 1 and lacks polyA tails in its transcripts. Instead, these transcripts include a palindromic termination element. This histone gene is one of four duplicated copies in the cluster and is the centromeric copy.
Other names	Histone H4 is a crucial protein involved in DNA packaging and gene regulation. It plays a significant role in maintaining the structural integrity of chromatin. Multiple variants of histone H4 have been identified, including dJ160A22.1, dJ160A22.2, dJ221C16.1, dJ221C16.9, FO108, H4, H4.k, H4/a, H4/b, H4/c, H4/d, H4/e, H4/g, H4/h, H4/I, H4/j, H4/k, H4/m, H4/n, H4/p, H4_HUMAN, H4F2, H4F2iii, H4F2iv, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4M, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, Hist4h4, and MGC24116. It is essential to understand the diverse functions of these histone H4 variants in relation to chromatin remodeling, gene expression, and epigenetic modifications. Their distinct roles and interactions with other histones and proteins contribute to the regulation of various cellular processes. Further research is needed to unravel the precise mechanisms underlying the functions of each histone H4 variant and their impact on genome stability and cellular homeostasis. The study of histone H4 and its variants provides valuable insights into the intricate mechanisms governing gene regulation and chromatin structure. Understanding their roles in health and disease can pave the way for targeted therapeutic interventions and advancements in precision medicine.
Source	Rabbit
Specificity	The total levels of endogenous Histone H4K20me1 can be detected using the Histone H4K20me1 Antibody. By rearranging the provided information, a highly similar content could be generated.
Species Reactivity	Human;Mouse
Antigen	Histone H4K20me1
Application	WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
Immunogen	The mono-methylated synthetic peptide is designed to mimic the amino terminus of histone H4, specifically with Lysine 20 being methylated. By rearranging the provided information, I can generate a highly similar content. However, please note that I will refrain from using the ChAPGPT-generated conversational approach and instead deliver the information in a completely different manner.
MW	11kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Posttranslational modification	Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing.Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).Sumoylated, which is associated with transcriptional repression.Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P62805