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Rabbit Anti-NEK3 Polyclonal Antibody#abs139657

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Description

Catalog-specification	Delivery time	USD price
abs139657-100ug	1-2 Weeks	301.0
abs139657-50ug	1-2 Weeks	201.0

Overview
catalog	abs139657
Other names	NEK3, also known as Never in mitosis A-related kinase 3 or NimA related protein kinase 3, is a serine/threonine-protein kinase that plays a vital role in cell division and cell cycle checkpoints. This protein belongs to the NIMA (never in mitosis gene a) related kinase family and is highly conserved in various organisms, including humans. NEK3 regulates the proper segregation of chromosomes during mitosis by phosphorylating microtubule-associated proteins. It is also involved in the regulation of centrosome duplication and the G2/M checkpoint. Dysregulation of NEK3 has been associated with the development and progression of several types of cancer. Therefore, NEK3 is a promising target for the development of anticancer therapies and the development of diagnostic tools for cancer detection.
Source	Rabbit
Specificity	Total NEK3 levels can be detected by using the NEK3 antibody, which is capable of identifying endogenous levels of NEK3. This allows for accurate identification and analysis of NEK3 in samples, providing valuable insight into biological processes and potential therapeutic targets.
Species Reactivity	Human;Mouse;Rat
Antigen	NEK3
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human NEK3.
MW	58 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized in the purification process of the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a maximum of one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing. This will ensure that the product remains effective and efficient for its intended use. It is important to follow these guidelines to ensure that the product continues to perform as expected and meets the highest quality standards.
Storage buffer	This solution contains rabbit IgG dissolved in phosphate buffered saline at a pH of 7.4, with the addition of 150 mM NaCl, 0.02% sodium azide and 50% glycerol. It is recommended that the solution be stored at -20 °C and it is stable for up to 12 months from the date of receipt.
Target
Background	Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.
Tissue specificity	Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.
Posttranslational modification	Phosphorylation at Thr-479 regulates its catalytic activity.
Celluar localization	Nucleus;
UniPort	P51956