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Rabbit Anti-F12 Polyclonal Antibody#abs136078

Please note that the provided price is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent. Western blot analysis of extracts of Jurkatcell lines, using F12 antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs136078-100ug	1-2 Weeks	301.0
abs136078-50ug	1-2 Weeks	201.0

Please note that the provided price is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent.

Overview
catalog	abs136078
Description	The gene responsible for producing coagulation factor XII is known for encoding a zymogen that exists in the bloodstream. Upon activation, the zymogen is cleaved to form a two-chain serine protease with both light and heavy chains. The heavy chain is comprised of various domains such as fibronectin-type, epidermal growth factor (EGF)-like, kringle, and proline-rich domains. In contrast, the light chain only contains a catalytic domain. Once activated, the heavy chain undergoes further cleavages to produce beta-factor XIIa light chain and convert alpha-factor XIIa light chain into beta-factor XIIa heavy chain. Through cleavage of prekallikrein, kallikrein is formed and subsequently cleaves factor XII into first alpha-factor XIIa and then beta-factor XIIa. The active factor XIIa plays a role in blood coagulation initiation, fibrinolysis, and the production of bradykinin and angiotensin. Additionally, it activates coagulation factors VII and XI. It's worth noting that defects in this gene don't produce any clinical symptoms, but solely prolong the whole-blood clotting time. This information was provided by RefSeq in July of 2008.
Other names	Factor XII, also known as Hageman factor, is an important component of the coagulation cascade. It is a crucial factor in initiating the intrinsic pathway of blood clotting. Within the coagulation process, Factor XII undergoes proteolytic activation, resulting in the formation of two active forms, Beta Factor XIIa part 1 and Beta Factor XIIa part 2. Beta Factor XIIa part 1 and Beta Factor XIIa part 2 are formed from the cleavage of Factor XII and consist of heavy and light chains. These chains play a significant role in the coagulation process, particularly in the activation of Factor XI, which subsequently leads to the production of thrombin and fibrin clot formation. Deficiency in Factor XII, also known as F12 deficiency or HAF deficiency, can lead to abnormal bleeding tendencies. HAE3, HAEX, and HAF are other names associated with Factor XII deficiency. This condition is inherited in an autosomal recessive manner, meaning that both parents must carry the defective gene for their child to develop the deficiency. In summary, Factor XII is an essential component of blood clotting, and its activation into Beta Factor XIIa part 1 and Beta Factor XIIa part 2 is critical for initiating the coagulation cascade. Deficiency in Factor XII can lead to bleeding disorders, highlighting its crucial role in hemostasis.
Source	Rabbit
Specificity	Endogenous levels of total F12 can be detected by the F12 Antibody. This antibody is highly effective in identifying the protein in question, and its use is applicable for various experimental situations. Whether in clinical or research settings, detection of F12 levels can be conveniently achieved with this antibody.
Species Reactivity	Human;Rat
Predictive reaction species	Rabbit;Horse;Pig
Antigen	F12
Application	For Western blotting, dilutions of 1:500 to 1:2000 are recommended. If using immunohistochemistry, dilutions of 1:50 to 1:200 should provide optimal results. When performing immunofluorescence or immunocytochemistry, it is suggested to use dilutions ranging from 1:100 to 1:500. For ELISA with a peptide antigen, a dilution of 1:20,000 to 1:40,000 is appropriate. Remember to adjust dilutions based on the specific experimental conditions, as they may vary depending on the antibody and antigen used.
Immunogen	A synthesized peptide derived from human F12.
MW	68kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.
Posttranslational modification	Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P00748