Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-S100A8 Polyclonal Antibody#abs136076

Please note that the price mentioned above is solely for your reference and may not reflect the actual price. For an accurate quote, we kindly advise that you get in touch with our dedicated seller, Vecent. Our team will gladly assist you with any further inquiries or concerns you may have about...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136076-100ug	1-2 Weeks	301.0
abs136076-50ug	1-2 Weeks	201.0

Overview
catalog	abs136076
Description	The S100 gene is responsible for encoding a protein that belongs to the S100 family. This protein contains two EF-hand calcium-binding motifs. S100 proteins are found in the cytoplasm and nucleus of various cells and play a role in regulating cellular processes like cell cycle progression and differentiation. The S100 gene cluster, which consists of at least 13 members, is located on chromosome 1q21. It is believed that this protein functions as both an inhibitor of casein kinase and a cytokine. Importantly, changes in the expression of this protein have been linked to the development of cystic fibrosis, a serious disease. This information has been provided by RefSeq in July 2008.
Other names	The protein S100 calcium binding protein A8, also known as calgranulin A, is encoded by the S100A8 gene. It is a member of the S100 family of calcium-binding proteins. S100A8 is involved in various cellular processes and is found in abundance in neutrophils and monocytes. One of the main functions of S100A8 is its role in inflammation. It is a pro-inflammatory protein that is released by activated immune cells in response to infection or injury. S100A8 acts as a chemotactic cytokine, attracting other immune cells to the site of inflammation. It also promotes the migration of immune cells across blood vessels. In addition to its role in inflammation, S100A8 is also involved in other biological processes. It has antimicrobial activity and can suppress the growth of certain bacteria and fungi. S100A8 is also implicated in the pathogenesis of several diseases. It is found at high levels in conditions like cystic fibrosis and urinary stone formation. S100A8 often forms a complex with another protein, S100A9, known as the S100A8/S100A9 complex or calprotectin. This complex has a range of functions and is involved in regulating immune responses and modulating inflammatory processes. Overall, S100 calcium binding protein A8 is a versatile protein that plays a crucial role in the immune system and inflammation. Its dysregulation can contribute to the development of various diseases, making it an important target for further research and therapeutic interventions.
Source	Rabbit
Specificity	The S100A8 Antibody is designed to recognize and measure the levels of total S100A8 that naturally exist within a biological sample. By utilizing this antibody, researchers can accurately detect and quantify the endogenous levels of S100A8 protein. It is an invaluable tool in various experimental and clinical settings, providing valuable insights into the expression and regulation of S100A8 in different biological systems.
Species Reactivity	Human;Mouse;Rat
Antigen	S100A8
Application	We have a range of dilution ratios for different applications. For Western blotting, the recommended dilutions are 1:500 to 1:2000. When performing immunohistochemistry, the dilutions range from 1:50 to 1:200. For immunofluorescence or immunocytochemistry, the suggested dilutions are 1:100 to 1:500. Lastly, for ELISA using a peptide substrate, the dilutions should be between 1:20000 and 1:40000. These dilution ratios are important for achieving optimal results in each respective assay.
Immunogen	A synthesized peptide derived from human S100A8.
MW	11kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. The iNOS-S100A8/A9 transnitrosylase complex directs selective inflammatory stimulus-dependent S-nitrosylation of GAPDH and probably multiple targets such as ANXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif; S100A8 seems to contribute to S-nitrosylation site selectivity.
Tissue specificity	Calprotectin (S100A8/9) is predominantly expressed in myeloid cells. Except for inflammatory conditions, the expression is restricted to a specific stage of myeloid differentiation since both proteins are expressed in circulating neutrophils and monocytes but are absent in normal tissue macrophages and lymphocytes. Under chronic inflammatory conditions, such as psoriasis and malignant disorders, also expressed in the epidermis. Found in high concentrations at local sites of inflammation or in the serum of patients with inflammatory diseases such as rheumatoid, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, cystic fibrosis, Sjogren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Involved in the formation and deposition of amyloids in the aging prostate known as corpora amylacea inclusions. Strongly up-regulated in many tumors, including gastric, esophageal, colon, pancreatic, bladder, ovarian, thyroid, breast and skin cancers.
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P05109