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Rabbit Anti-EMD Polyclonal Antibody#abs135786

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Description

Catalog-specification	Delivery time	USD price
abs135786-100ug	1-2 Weeks	301.0
abs135786-50ug	1-2 Weeks	201.0

Overview
catalog	abs135786
Description	Emerin is an important integral protein found in the nuclear inner membrane, and its expression is widespread throughout various cells (1). This protein plays a crucial role in nuclear functions and interacts with various nuclear proteins, including BAF and A- and B-type lamins (2-5). The presence of a LEM domain in Emerin allows it to bind with these proteins, contributing to their regulation and maintenance of nuclear integrity. Furthermore, Emerin's involvement in gene expression regulation is noteworthy, as it can interact with other transcriptional regulators (6,7). By doing so, Emerin potentially influences the expression of various genes, thereby impacting cellular processes. Additionally, it has been observed that Emerin has the ability to bind to β-catenin, inhibiting its accumulation in the nucleus (8). This interaction between Emerin and β-catenin could have significant implications for cellular signaling pathways and potentially affect various physiological processes. In recent studies, it has been discovered that Emerin plays a vital role in HIV-1 infectivity (9). The exact mechanisms by which Emerin contributes to this process are yet to be fully understood, but its involvement suggests that it has implications beyond nuclear functions. Importantly, mutations in Emerin are responsible for a major form of muscular dystrophy called Emery-Dreifuss muscular dystrophy (EDMD) (10). This disorder is characterized by progressive weakening of skeletal muscles, and understanding the role of Emerin in this condition is crucial for developing effective treatment strategies. In conclusion, Emerin's broad expression and its interactions with various nuclear proteins demonstrate its importance in maintaining nuclear functions and regulating gene expression. Its involvement in HIV-1 infectivity and its association with EDMD highlight the diverse roles this integral protein plays within cellular processes. Further research on Emerin will undoubtedly provide valuable insights into the complexities of nuclear biology and associated diseases.
Other names	Emery Dreifuss muscular dystrophy (EDMD) is a genetic disorder characterized by the mutations in the emerin gene (EMD). This condition primarily affects skeletal muscles, causing progressive muscle wasting and weakness. Individuals with EDMD may experience joint stiffness, contractures, and cardiac abnormalities. The emerin protein, encoded by the EMD gene, plays a crucial role in maintaining the structural integrity of the nucleus and regulating gene expression. Understanding the underlying mechanisms of EDMD can help develop potential therapeutic strategies to improve the quality of life for affected individuals.
Source	Rabbit
Specificity	The EMD Antibody is capable of recognizing the complete natural levels of EMD within an organism.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;
Antigen	EMD
Application	The recommended dilution for Western blotting is between 1:500 to 1:2000, while for immunohistochemistry it is between 1:50 to 1:200. For ELISA (peptide), a dilution of 1:20000 to 1:40000 is suggested. It's important to note that these dilutions may need to be optimized depending on the specific assay and sample type.
Immunogen	A synthesized peptide derived from human EMD.
MW	29kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. Our process ensured that the resulting product was highly pure and of top-notch quality.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. Required for proper localization of non-farnesylated prelamin-A/C.
Tissue specificity	Skeletal muscle, heart, colon, testis, ovary and pancreas.
Posttranslational modification	Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
Celluar localization	Cytoskeleton;Endoplasmic reticulum;Nucleus;
UniPort	P50402