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Rabbit Anti-FBL Polyclonal Antibody#abs135790

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent. Western blot analysis of extracts of various celllines, using FBL antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs135790-100ug	1-2 Weeks	301.0
abs135790-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For detailed pricing information, please get in touch with our seller, Vecent.

Overview
catalog	abs135790
Description	Fibrillarin is an enzyme that plays a crucial role in RNA transcription and pre-RNA processing within the nucleolus. It is primarily located in the fibrillar regions and Cajal bodies of the nucleolus, where it associates with other structural proteins and box C/D snoRNA to form a complex that is involved in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. The complex, which is catalyzed by fibrillarin, is responsible for the site-specific 2'-O-ribose methylation of specific nucleotides within the rRNA sequence. This process is critical for the proper functioning of the ribosome, which is responsible for protein synthesis within the cell. Fibrillarin is highly conserved, both in terms of its sequence and its function, and its expression is essential for early embryonic development. Without fibrillarin, the proper processing and functioning of pre-rRNA would be compromised, leading to developmental defects and potentially fatal outcomes. Overall, fibrillarin plays a vital role in the overall functioning of the nucleolus and its associated processes, and its importance cannot be overstated in terms of its contribution to proper RNA processing and ribosome assembly.
Other names	Fibrillarin, also known as 34 kD nucleolar scleroderma antigen or Fbl, is an essential protein involved in various cellular processes. It is encoded by the FBRL_HUMAN gene and is crucial for the methylation of histone-glutamine residues. Fibrillarin also interacts with RNA U3 small nucleolar interacting protein 1 (RNU3IP1) and functions as an rRNA 2'-O-methyltransferase. The 34 kDa nucleolar scleroderma antigen is a significant component of the nucleolus, a specialized subcompartment of the cell nucleus. It plays a vital role in the modification of ribosomal RNA (rRNA) by catalyzing the addition of a methyl group to the 2'-OH position of specific nucleotides. This process is essential for the proper maturation and function of rRNA. Fibrillarin, or Fbl, is involved in the assembly of small nucleolar ribonucleoproteins (snoRNPs) and the processing of precursor rRNA. It localizes to the nucleolus, where it interacts with other proteins and RNA molecules to carry out its functions. Fibrillarin is essential for cell growth and development, and its dysfunction is associated with various diseases, including scleroderma. In summary, fibrillarin is a multifunctional protein that plays a critical role in RNA modification and processing. Its diverse functions make it an indispensable component of cellular machinery, highlighting its importance in various biological processes.
Source	Rabbit
Specificity	The FBL Antibody has the ability to identify the total FBL present in the body. Its detection mechanism is based on the recognition of endogenous levels of FBL. To create a highly similar content, one might rearrange the above statement and state that the FBL Antibody detects the total FBL present in the system by identifying its endogenous levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Sheep;Dog;Bovine;Horse
Antigen	FBL
Application	The recommended dilutions for our antibody products are as follows: for Western blotting, use a dilution range from 1:500 to 1:2000; for immunohistochemistry, use a dilution range from 1:50 to 1:200; and for ELISA (peptide), use a dilution range from 1:20000 to 1:40000. By following these dilution guidelines, you can achieve optimal results in your experiments and ensure the best possible performance of our antibodies. So, be sure to use the appropriate dilution for your specific application to obtain accurate and reliable results every time.
Immunogen	A synthesized peptide derived from human FBL.
MW	34kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
Posttranslational modification	By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P22087