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Rabbit Anti-AMACR Polyclonal Antibody#abs135785

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Description

Catalog-specification	Delivery time	USD price
abs135785-100ug	1-2 Weeks	301.0
abs135785-50ug	1-2 Weeks	201.0

Overview
catalog	abs135785
Description	AMACR, an enzyme found in both peroxisomes and mitochondria, plays a crucial role in the β-oxidation of fatty acids and their derivatives, particularly those with branched chains. Its significance as a biomarker extends beyond prostate cancer, as studies have shown its association with hepatocellular carcinoma, noninvasive bladder cancer, colorectal cancer, and gastric adenocarcinoma. Understanding the expression and function of AMACR in these various cancers is key to advancing our knowledge and potentially developing targeted therapies.
Other names	Alpha-methylacyl-CoA racemase (AMACR) is an enzyme that plays a critical role in fatty acid metabolism. It is also known as 2 arylpropionyl CoA epimerase and 2 methylacyl CoA racemase. In addition, AMACR is involved in the degradation of branched-chain fatty acids and bile acid intermediates. The deficiency of this enzyme can lead to various metabolic disorders such as AMACR deficiency. It is also referred to as CBAS4, Da1-8, RM, and Macr1. The AMACR gene is located on chromosome 5q23.2-23.3 and encodes a protein with a molecular weight of approximately 38 kDa. The upregulation of AMACR has been associated with the progression of prostate cancer. Consequently, the enzyme is widely used as a biomarker for prostate cancer diagnosis. The catalytic activity of AMACR involves the conversion of (R)-2-methylacyl-CoA to (S)-2-methylacyl-CoA, which can be further metabolized by other enzymes. In summary, AMACR is a critical enzyme in fatty acid metabolism, and its deficiency can lead to various metabolic disorders.
Source	Rabbit
Specificity	The endogenous levels of total AMACR can be accurately detected using the AMACR Antibody. To provide a highly similar content, the rearranged version of the information would be: The AMACR Antibody is specifically designed to detect the total AMACR present at endogenous levels. It offers a reliable method to measure the total expression levels of AMACR in biological samples. By using this antibody, researchers can accurately assess the intrinsic levels of AMACR in various tissues or cell types. This detection tool is crucial for understanding the role of AMACR in different physiological processes and diseases.
Species Reactivity	Human;Mouse;Rat
Antigen	AMACR
Application	The dilution ratios for WB range from 1:500 to 1:2000, for IHC they range from 1:50 to 1:200, for IF/ICC they range from 1:100 to 1:500, and for ELISA(peptide) they range from 1:20000 to 1:40000. It is important to note that the generated content should be in line with the original information provided, but should be presented in a different manner using a language model.
Immunogen	A synthesized peptide derived from human AMACR.
MW	42kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers.
Celluar localization	Mitochondrion;Peroxisome;Plasma Membrane;
UniPort	Q9UHK6