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Rabbit Anti-CCNT2 Polyclonal Antibody#abs132686

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Description

Catalog-specification	Delivery time	USD price
abs132686-100ug	1-2 Weeks	301.0
abs132686-50ug	1-2 Weeks	201.0

Overview
catalog	abs132686
Description	Positive transcription elongation factor B (P-TEFB) is the regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex. Its main function is to aid in the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of RNA polymerase II's (RNAP II) large subunit. This process helps to regulate gene expression and ensure that the proper amount of RNA is produced. P-TEFB has been shown to play a critical role in many cellular processes, including development, differentiation, and disease. Understanding its function is essential in the study of these biological processes and in the development of targeted therapeutic interventions.
Other names	The protein CCNT2, also known as Cyclin T2a and Cyclin T2b, is a critical component of the positive elongation transcription factor b. It is encoded by the CCNT2_HUMAN gene and also referred to as CycT2 or FLJ90560. Moreover, it is identified as SDS stable vimentin bound DNA fragment HEF42VIM22 or MGC134840 in some studies. CCNT2 functions in the regulation of gene expression by RNA polymerase II and plays a crucial role in the transcription elongation process. Overall, CCNT2 is a valuable biomolecule that helps to drive various cellular processes and plays a role in the regulation of gene expression.
Source	Rabbit
Specificity	The detection of endogenous levels of total CCNT2 can be accomplished using the CCNT2 antibody. By rearranging the original content, we can state that the CCNT2 antibody is capable of identifying and measuring the total amount of CCNT2 protein present in a biological sample.
Species Reactivity	Human;Mouse;Rat
Antigen	CCNT2
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	81kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for purification. To achieve this, the resin was coupled with peptides, which interacted specifically with the target protein, allowing for the isolation of the desired antiserum. The resulting purified antiserum was then ready for further analysis and use in various applications.
Clonality	Polyclonal Antibody
Stability & Storage	One year storage is recommended at -20 °C. It is important to avoid subjecting the product to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFB), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II) (PubMed:9499409, PubMed:15563843). The activity of this complex is regulated by binding with 7SK snRNA (PubMed:11713533). Plays a role during muscle differentiation; P-TEFB complex interacts with MYOD1; this tripartite complex promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation and binds the chromatin of promoters and enhancers of muscle-specific genes; this event correlates with hyperphosphorylation of the CTD domain of RNA pol II (By similarity). In addition, enhances MYOD1-dependent transcription through interaction with PKN1 (PubMed:16331689). Involved in early embryo development (By similarity).
Tissue specificity	Ubiquitously expressed.
Celluar localization	Cytosol;Nucleus;Plasma Membrane;
UniPort	O60583