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Rabbit Anti-EGLN2 Polyclonal Antibody#abs137395

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Description

Catalog-specification	Delivery time	USD price
abs137395-100ug	1-2 Weeks	301.0
abs137395-50ug	1-2 Weeks	201.0

Overview
catalog	abs137395
Other names	Egl Nine (C.elegans) Homolog 2, also known as EGLN2 or Estrogen-Induced Tag 6, is a protein encoded by the DKFZp434E026 gene. It is a homolog of EGL nine in the nematode Caenorhabditis elegans. EGLN2 is also referred to as HIF-PH1, HPH-1, or Prolyl Hydroxylase Domain-Containing Protein 1. It plays a crucial role in the regulation of hypoxia-inducible factor (HIF), a protein involved in cellular response to low oxygen levels. EGLN2 helps in the hydroxylation of specific proline residues in HIF, leading to its degradation under normoxic conditions. It is an important factor in oxygen homeostasis and has implications in various physiological and disease processes.
Source	Rabbit
Specificity	The EGLN2 Antibody is capable of detecting total EGLN2 levels within the body. This includes levels that are naturally occurring within the body without external interference. By using this antibody, researchers can accurately assess the levels of EGLN2 without influencing the body's natural state.
Species Reactivity	Human;Mouse;Rat
Antigen	EGLN2
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human EGLN2.
MW	44 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality and stability of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing. By following these guidelines, you can ensure that the product remains effective and reliable for its intended use.
Storage buffer	This Rabbit IgG solution is formulated in phosphate buffered saline at a neutral pH of 7.4, with 150mM NaCl and 0.02% sodium azide added as preservatives. It also contains 50% glycerol for enhanced stability, and should be stored at a temperature of -20 °C. This product is known to maintain its quality for a period of 12 months from the date of receipt.
Target
Background	Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferentially recognized via a LXXLAP motif.
Tissue specificity	Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
Celluar localization	Nucleus;
UniPort	Q96KS0