Rabbit Anti-DNMT3B Polyclonal Antibody#abs131595
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Description
| Catalog-specification | Delivery time | USD price |
abs131595-50ug | 1-2 Weeks | 201.0 |
abs131595-100ug | 1-2 Weeks | 301.0 |
Kindly note that the price mentioned here is only for your reference. To obtain the detailed price, please get in touch with our seller Vecent. We would like to emphasize that the content generated through language modeling will differ significantly from the ChapGPT generated content. Therefore, we advise you to rely on the original text information provided to deliver your message effectively.
| Overview | |
catalog | abs131595 |
Description | For the establishment of DNA methylation patterns during development, genome wide de novo methylation is imperative. This process is tightly coordinated with methylation of histones and may show preference for the nucleosomal DNA within the nucleosome core region. Additionally, it is essential to have this process to ensure proper regulation of gene expression and to maintain the stability and integrity of the genome. The importance of DNA methylation cannot be overstated and it plays a pivotal role in coordinating a wide range of biological processes. Therefore, understanding the intricate mechanisms involved in this process remains crucial for advancing our knowledge of developmental biology and disease pathogenesis. |
| Other names | Cytosine 5-methyltransferase 3B, also known as DNA (cytosine 5) methyltransferase 3 beta, is an enzyme involved in DNA methylation. It plays a crucial role in adding methyl groups to cytosine bases of DNA molecules. DNA methyltransferase HsaIIIB, or Dnmt3b, is another name for this enzyme. Its official gene symbol is DNM3B_HUMAN and it is also known as ICF1 or M.HsaIIIB. The protein is encoded by the RP23-89H14.3 gene and is also referred to as MGC124407. This enzyme belongs to the EC 2.1.1.37 enzyme class. It is associated with a genetic disorder called Immunodeficiency, Centromeric region instability, and Facial anomalies syndrome (ICF). This information highlights the important role of Cytosine 5-methyltransferase 3B in DNA methylation and its relevance in certain genetic conditions. |
| Source | Rabbit |
| Specificity | The detection of endogenous levels of total DNMT3B is facilitated by the DNMT3B Antibody. The content is rearranged to emphasize the antibody's capability. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | DNMT3B |
| Application | The dilution ranges for WB, IHC, IF, and ELISA (peptide) are as follows: - WB: 1:500-1:2000 - IHC: 1:50-1:200 - IF: 1:100-1:500 - ELISA (peptide): 1:20000-1:40000 To ensure accurate results, it is crucial to dilute the samples within these ranges. For WB, the optimal dilution is between 1:500 and 1:2000. Similarly, for IHC, the recommended dilution is between 1:50 and 1:200. IF assays should be performed with samples diluted between 1:100 and 1:500. Finally, for ELISA (peptide) experiments, a dilution range of 1:20000 to 1:40000 is suggested. Careful consideration should be given to these dilution ranges to achieve reliable and reproducible outcomes in scientific research. Dilutions outside these recommended ranges may lead to inaccurate or inconclusive results. Hence, it is important to adhere to the suggested dilution guidelines for each assay to obtain meaningful data. |
| Immunogen | A synthesized peptide derived from human DNMT3B. |
| MW | 97 kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Peptide affinity chromatography using SulfoLink™ Coupling Resin was utilized for the purification of the antiserum. The purification process was successful in isolating the desired components of the antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure optimal preservation, it is recommended that the product be kept at a temperature of -20 °C and stored for up to one year. It is advisable to avoid subjecting the product to repeated cycles of freezing and thawing, as this could impact its quality and efficacy. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398). |
| Tissue specificity | Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. |
| Posttranslational modification | Sumoylated.Citrullinated by PADI4. |
| Celluar localization | Nucleus; |
| UniPort | Q9UBC3 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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