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Rabbit Anti-EPHA4 Polyclonal Antibody#abs131597

Description

Catalog-specification	Delivery time	USD price
abs131597-50ug	1-2 Weeks	201.0
abs131597-100ug	1-2 Weeks	301.0

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Overview
catalog	abs131597
Description	Adjacent cells are engaged in contact-dependent bidirectional signaling through a receptor tyrosine kinase that binds to membrane-bound ephrin family ligands. This receptor initiates forward signaling, while the ephrin ligand triggers reverse signaling downstream.
Other names	The protein receptor known as Cek 8 or Ephrin type-A receptor 4 is also referred to as Eph-like kinase 8 and TYRO1. It belongs to the family of eph receptors, which are crucial for various cellular functions and signaling pathways. This particular receptor, EPHA4, is involved in embryonic development, synaptic plasticity, and other processes related to the nervous system. HEK 8 is also a name for this protein, as well as Sek 1 and Receptor protein-tyrosine kinase HEK8. This tyrosine-protein kinase is important for cell growth and differentiation, and aberrations in its function are associated with diseases such as cancer and neurodegeneration. Understanding the complex interplay and regulation of the different eph receptors and their ligands is an active area of research, with potential implications for therapeutic interventions in various diseases.
Source	Rabbit
Specificity	The EPHA4 Antibody has the ability to identify total endogenous levels of EPHA4. This implies that it can accurately detect the presence of all EPHA4 molecules within the sample being analyzed.
Species Reactivity	Human;Mouse;Rat
Antigen	EPHA4
Application	The dilutions for Western blot (WB) range from 1:500 to 1:2000, for immunohistochemistry (IHC) they range from 1:50 to 1:200, and for ELISA using a peptide they range from 1:20000 to 1:40000. It is important to note that the generated content will be different from the original text information, as per the given instruction.
Immunogen	A synthesized peptide derived from human EPHA4.
MW	110 kd
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized to purify the antiserum through peptide affinity chromatography. This process yielded highly purified antiserum for use in further experimentation.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.
Tissue specificity	Ubiquitous.
Celluar localization	Endoplasmic reticulum;Endosome;Golgi apparatus;Mitochondrion;Plasma Membrane;
UniPort	P54764