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Rabbit Anti-CFB Polyclonal Antibody#abs136087

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts of various celllines, using CFB antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs136087-100ug	1-2 Weeks	301.0
abs136087-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs136087
Description	Complement factor B, encoded by this gene, plays a crucial role in the alternative pathway of complement activation. It is found in the bloodstream as a single chain polypeptide. When the alternative pathway is activated, complement factor D cleaves it, resulting in the formation of two subunits: Ba and Bb. Bb acts as a serine protease and combines with C3b to form the alternative pathway C3 convertase. This active subunit, Bb, promotes the proliferation of preactivated B lymphocytes, while Ba restricts their proliferation. The gene is located in the major histocompatibility complex (MHC) class III region on chromosome 6, which encompasses various genes involved in regulating immune reactions. Interestingly, variations in this gene have been linked to a decreased risk of age-related macular degeneration. Notably, the polyadenylation site of this gene is positioned 421 bp away from the 5' end of the gene responsible for complement component 2.
Other names	The C3/C5 convertase is a key player in the complement system, which is an important part of the immune system. Complement factor B is an essential component of this convertase complex. Another important protein involved in this process is the glycine-rich beta glycoprotein, also known as PBF2. Finally, properdin factor B is another critical component of the complement system. Together, these proteins work to help the body fight off infections and other threats.
Source	Rabbit
Specificity	The detection capability of CFB Antibody extends to the endogenous levels of total CFB, allowing for accurate analysis of CFB protein present in a biological sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	CFB
Application	The dilutions for WB are 1:500-1:2000, for IHC are 1:50-1:200, for IF/ICC are 1:100-1:500, and for ELISA (peptide) are 1:20000-1:40000. It is important to note that these dilutions are based on the original text information provided.
Immunogen	A synthesized peptide derived from human CFB.
MW	86kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P00751