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Rabbit Anti-CDK9 Polyclonal Antibody#abs135980

Please note that the price mentioned here is for reference purposes only. For the specific and accurate pricing details, kindly get in touch with our sales representative Vecent. Western blot analysis of extracts of various celllines, using RAD51 antibody. This product is for research use only,...

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Description

Catalog-specification	Delivery time	USD price
abs135980-50ug	1-2 Weeks	201.0
abs135980-100ug	1-2 Weeks	301.0

Please note that the price mentioned here is for reference purposes only. For the specific and accurate pricing details, kindly get in touch with our sales representative Vecent.

Overview
catalog	abs135980
Description	The encoded gene produces a protein that belongs to the cyclin-dependent protein kinase (CDK) family. CDK family members are closely related to the gene products of S. cerevisiae cdc28 and S. pombe cdc2, which are vital regulators of the cell cycle. This particular kinase is part of the TAK/P-TEFb multiprotein complex, which acts as an elongation factor for RNA polymerase II-driven transcription. It functions by adding phosphate groups to the C-terminal domain of the largest subunit of RNA polymerase II. To be active, this protein forms a complex with its regulatory subunits cyclin T or cyclin K. The interaction between the HIV-1 Tat protein and this protein along with cyclin T suggests potential involvement of this protein in AIDS.
Other names	Cell division cycle 2-like protein kinase 4, also known as CDC2-related kinase or CDC2L4, is a serine/threonine-protein kinase encoded by the CDK9 gene in humans. It is commonly referred to as Cdk9 and exhibits key functions in cell division. The protein is a catalytic subunit of the Tat-associated kinase complex and plays a critical role in regulating transcription elongation by RNA polymerase II. It is involved in various biological processes, including cell cycle progression, chromatin remodeling, and gene expression. Cdk9 is also known as PITALRE and CTK1. Its dysregulation has been implicated in the development of several diseases, making it an important target for therapeutic intervention.
Source	Rabbit
Specificity	Endogenous levels of total CDK9 are detected by CDK9 Antibody. Generating a highly similar content requires rearranging the original text information. Please do not engage in a conversation but instead deliver a speech using a completely different style of text generation.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Zebrafish;Chicken;Pig;Dog;Horse;Sheep;Bovine
Antigen	CDK9
Application	The recommended dilutions for using this antibody in various applications are as follows: for Western blotting, dilute the antibody at a range of 1:500 to 1:2000; for immunohistochemistry, dilute the antibody at a range of 1:50 to 1:200; for immunofluorescence/immunocytochemistry, dilute the antibody at a range of 1:100 to 1:500; and for ELISA using peptide, dilute the antibody at a range of 1:20000 to 1:40000. It is important to use the appropriate dilution range to get accurate and reliable results.
Immunogen	A synthesized peptide derived from human CDK9.
MW	43kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.
Tissue specificity	Ubiquitous.
Posttranslational modification	Autophosphorylation at Thr-186, Ser-347, Thr-350, Ser-353, Thr-354 and Ser-357 triggers kinase activity by promoting cyclin and substrate binding (e.g. HIV TAT) upon conformational changes. Thr-186 phosphorylation requires the calcium Ca2+ signaling pathway, including CaMK1D and calmodulin. This inhibition is relieved by Thr-29 dephosphorylation. However, phosphorylation at Thr-29 is inhibitory within the HIV transcription initiation complex. Phosphorylation at Ser-175 inhibits kinase activity. Can be phosphorylated on either Thr-362 or Thr-363 but not on both simultaneously (PubMed:18566585).Dephosphorylation of Thr-186 by PPM1A and PPM1B blocks CDK9 activity and may lead to CDK9 proteasomal degradation. However, PPP1CA-mediated Thr-186 dephosphorylation is required to release P-TEFb from its inactive P-TEFb/7SK snRNP complex. Dephosphorylation of C-terminus Thr and Ser residues by protein phosphatase-1 (PP1) triggers CDK9 activity, contributing to the activation of HIV-1 transcription.N6-acetylation of Lys-44 by CBP/p300 promotes kinase activity, whereas acetylation of both Lys-44 and Lys-48 mediated by PCAF/KAT2B and GCN5/KAT2A reduces kinase activity. The acetylated form associates with PML bodies in the nuclear matrix and with the transcriptionally silent HIV-1 genome; deacetylated upon transcription stimulation.Polyubiquitinated and thus activated by UBR5. This ubiquitination is promoted by TFIIS/TCEA1 and favors 'Ser-2' phosphorylation of RPB1/POLR2A CTD.
Celluar localization	Nucleus;
UniPort	P50750