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Rabbit Anti-HK1 Polyclonal Antibody#abs135719

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Description

Catalog-specification	Delivery time	USD price
abs135719-50ug	1-2 Weeks	201.0
abs135719-100ug	1-2 Weeks	301.0

Overview
catalog	abs135719
Description	Glycolysis, the metabolic pathway responsible for breaking down glucose, is initiated by hexokinase. Within mammals, there are four distinct isoforms of hexokinase: hexokinase I, II, III, and IV (also known as glucokinase). Hexokinases I, II, and III are primarily found on the outer mitochondrial membrane and play a crucial role in facilitating increased aerobic glycolysis in cancer cells. This metabolic phenomenon, known as the Warburg Effect, assists in meeting the heightened energy demands associated with the rapid growth and proliferation of these cells.
Other names	Hexokinase is an enzyme known to exist in different forms, such as brain form hexokinase, Hexokinase 1, and Hexokinase type I. It is also referred to as HK I, HK1 ta, HK1 tb, HK1 tc, HKI, or HXK1. This enzyme plays a vital role in catalyzing the phosphorylation of glucose to glucose-6-phosphate in the presence of ATP. By rearranging the given content, the generated text can be presented in a different manner by emphasizing the significance and diverse names of this enzyme.
Source	Rabbit
Specificity	Endogenous levels of total HK1 can be accurately detected with the HK1 Antibody. To reiterate, this antibody specifically recognizes endogenous HK1 levels.
Species Reactivity	Human;Mouse;Rat
Antigen	HK1
Application	We recommend dilutions for various applications as follows: for Western blotting, use a dilution range of 1:500-1:2000; for immunohistochemistry, dilute at 1:50-1:200; for immunofluorescence/immunocytochemistry, dilute at 1:100-1:500; and for peptide-based ELISA, dilution range of 1:20000-1:40000. These dilutions should provide optimal staining and detection results for your experiments. Please note that these recommendations are based on the original information provided.
Immunogen	A synthesized peptide derived from human HK1.
MW	102kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography employing SulfoLink™ Coupling Resin was utilized to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Tissue specificity	Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific.
Celluar localization	Cytosol;Mitochondrion;
UniPort	P19367