Rabbit Anti-GADD153 Polyclonal Antibody #abs130472

abs130472-50ug

1-2 Weeks

201

abs130472-100ug

1-2 Weeks

301

Description

CHOP, a member of the bZIP family of transcriptional-regulatory proteins, plays a crucial role in inhibiting the DNA-binding ability of C/EBP and LAP. This inhibition occurs by forming heterodimers that are incapable of binding DNA. It is believed to have significant involvement in the progression of melanoma. CK2-mediated phosphorylation of CHOP effectively hampers its transcriptional activity. Additionally, CHOP enhances the transcription of IL-6 by capturing the negative regulatory NF-IL6 isoform. Therefore, CHOP's functional repertoire encompasses both the suppression of certain transcription factors and the promotion of others, ultimately influencing gene expression patterns.

Other names

The protein known as C/EBP homologous protein, or CHOP, has several alternate names, including C/EBP homology protein, C/EBP zeta, C/EBP-homologous protein 10, and CCAAT/enhancer binding protein homologous protein. Additionally, it is sometimes referred to by its gene name, DDIT3, or as the DNA damage-inducible transcript 3 protein. This protein has also been given the names growth arrest and DNA damage inducible protein 153, or GADD153, and growth arrest and DNA damage-inducible protein, depending on the context in which it is being studied. Regardless of what name it goes by, CHOP plays a critical role in various cellular processes and has been extensively studied in both healthy and diseased organisms. For example, CHOP is known to be involved in cell differentiation, growth arrest, and apoptosis, the process of programmed cell death. It has also been implicated in a range of diseases, including cancer, neurodegeneration, and diabetes. Given its multifunctional roles and its involvement in such diverse pathologies, CHOP continues to be an area of active research in the scientific community.

Source

Rabbit

Specificity

The GADD153 Antibody is capable of detecting the natural levels of GADD153 present in the body. This antibody is specifically designed to target endogenous GADD153 and can be used to study its expression and function. By recognizing and binding to GADD153, this antibody allows researchers to determine its role in different cellular processes. Overall, the GADD153 Antibody is a valuable tool for investigating the function of this important protein in various biological systems.

Species Reactivity

Human;Mouse;Rat

Application

According to the original text information, the dilution ratios for Western blotting range from 1:500 to 1:2000. For immunohistochemistry (IHC), the recommended dilution ratios vary from 1:50 to 1:200. When it comes to immunofluorescence/immunocytochemistry (IF/ICC), the suitable dilution ratios range from 1:100 to 1:500. Lastly, for ELISA using peptide detection, the recommended dilution ratios are between 1:20000 and 1:40000.

Immunogen

A synthesized peptide derived from human GADD153.

Properties

Concentration

1mg/ml

Purification

SulfoLink™ Coupling Resin was employed in purifying the antiserum through peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can have a negative impact on its efficacy. Please ensure that the product is stored in a suitable location that meets the recommended temperature requirements to ensure its longevity.

Storage buffer

To ensure the stability and integrity of Rabbit IgG, it should be stored at a temperature of -20°C with a storage buffer of phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. This antibody can remain stable for up to 12 months from the date of receipt, giving users ample time to conduct their research experiments.

Target

Background

The ER stress response is regulated by a multifunctional transcription factor that plays a crucial role in various cellular stresses. It can induce cell cycle arrest and apoptosis when the ER is stressed. This transcription factor serves a dual purpose as it inhibits the function of CCAAT/enhancer-binding protein (C/EBP) and activates other genes. By dimerizing with C/EBP family members, it prevents their association with C/EBP binding sites in promoter regions, effectively suppressing the expression of C/EBP regulated genes.
On the positive side, this transcription factor promotes the transcription of several genes including TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM, and ERO1L. In contrast, it negatively regulates the expression of BCL2 and MYOD1, ATF4-dependent activation of asparagine synthetase (ASNS), CEBPA-dependent activation of hepcidin (HAMP), and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG).
Furthermore, this transcription factor inhibits the canonical Wnt signaling pathway by interacting with TCF7L2/TCF4, thereby impairing its DNA-binding ability and repressing its transcriptional activity. It also plays a role in regulating the inflammatory response by inducing caspase-11 (CASP4/CASP11), which in turn activates caspase-1 (CASP1). Both caspases enhance the activation of pro-IL1B, leading to the maturation of IL1B, a key molecule involved in the inflammatory response.
In summary, this multifunctional transcription factor in the ER stress response acts as both an inhibitor and activator of genes, exerting a significant impact on cell function and response to stress. Its complex regulatory role extends to the modulation of signaling pathways and inflammatory processes.

Tissue specificity

By oxidative stress, amino-acid deprivation, hypoxia and ER stress. During ER stress, induced by a EIF2AK3/ATF4 pathway and/or ERN1/ATF6 pathway. Expression is suppressed by TLR-TRIF signaling pathway during prolonged ER stress.

Posttranslational modification

Ubiquitinated, leading to its degradation by the proteasome.Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.

Celluar localization

Cytosol;Endosome;Nucleus;

UniPort

P35638