Rabbit Anti-CD63 Polyclonal Antibody#abs131194

abs131194-50ug

1-2 Weeks

201

abs131194-100ug

1-2 Weeks

301

Description

The role of this protein is to function as a cell surface receptor for TIMP1 and facilitate the activation of cellular signaling cascades. It is also involved in activating ITGB1 and integrin signaling, ultimately leading to the activation of AKT, FAK/PTK2, and MAP kinases. By participating in the activation of AKT and FAK/PTK2, it facilitates cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading, and migration. Moreover, it plays a crucial role in VEGFA signaling by regulating the internalization of KDR/VEGF.

Other names

The protein LAMP3, also known as CD63 or melanoma 1 antigen, is a glycoprotein that is mainly found in lysosomal membranes. It is encoded by the CD63 gene in humans, and is also known by various aliases such as MLA1, NGA, and PTLGP40. CD63 is a member of the tetraspanin superfamily, which includes proteins that are involved in multiple cellular functions such as signaling and adhesion.
CD63 has been identified as a marker for various cell types such as mast cells, platelets, and endothelial cells. It has also been implicated in a variety of physiological and pathological processes including antigen presentation, cell migration, and cancer metastasis.
Research has shown that CD63 interacts with other membrane proteins and participates in the regulation of lysosomal membrane trafficking and fusion. It has also been reported that CD63 is involved in the uptake and degradation of extracellular material such as exosomes and bacteria.
Overall, CD63 is a multifunctional protein with crucial roles in lysosomal function, membrane dynamics, and cellular homeostasis. Its diverse functions make it an interesting target for future research into the underlying mechanisms of cell behavior and disease progression.

Source

Rabbit

Specificity

The CD63 antibody is capable of detecting high levels of endogenous CD63 protein in various samples. This particular antibody is designed to recognize and bind to the total CD63 protein, providing a reliable method for studying the expression and function of CD63 in both research and diagnostic settings.

Application

The recommended dilutions for immunohistochemistry range from 1:50 to 1:200, while for Western blotting it is suggested to use a dilution of 1:1000. For ELISA using a peptide substrate, the recommended dilution is between 1:20000 and 1:40000. It is important to follow the recommended dilutions to ensure accurate and reliable results in your experiments.

A synthesized peptide derived from human CD63.

Properties

1mg/ml

Purification

SulfoLink™ Coupling Resin was utilized to perform peptide affinity chromatography, resulting in the purification of the antiserum. Let me know if you need further information.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20°C for up to one year. It is important to avoid exposing the product to repeated cycles of freezing and thawing. This can negatively affect its overall effectiveness, so proper storage is crucial. Thank you for choosing our product and we hope it meets your needs.

Storage buffer

The rabbit IgG is suspended in a solution of phosphate buffered saline with pH 7.4, 150mM NaCl, and 0.02% sodium azide. Additionally, it contains 50% glycerol and should be stored at -20°C. The product is stable for up to 12 months from the date of receipt, ensuring that it can be used effectively for a lengthy period without deterioration.

Target

Background

 Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Tissue specificity

Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.

Celluar localization

Endosome;Extracellular region or secreted;Lysosome;Plasma Membrane;

P08962