Phospho-RelB (Ser552) Rabbit Polyclonal Antibody

abs131005-50ug

1-2 Weeks

201

abs131005-100ug

1-2 Weeks

301

Description

RelB is a vital transcription factor in the nuclear factor-kappaB (NFkB) transcription complex. Its presence stimulates promoter activity together with NFkB1/NFkB-p50. Apart from that, RelB plays a crucial role in the regulation of apoptosis in hepatocellular carcinoma by activating downstream target genes. The overall significance and contribution of RelB cannot be overstated, especially in the context of cellular regulation and molecular processes. Its unique positioning in the NFkB complex makes it a key factor in the complex's functioning mechanisms, making a vast difference in the way the cell responds to stimuli and external factors.

Other names

I REL; I-Rel; IREL; Nuclear factor of kappa light polypeptide gene enhancer in B cells 3; relB; RELB_HUMAN; Reticuloendotheliosis viral oncogene homolog B; Transcription factor Rel B; Transcription factor RelB; v rel avian reticuloendotheliosis viral oncogene homolog B; v rel reticuloendotheliosis viral oncogene homolog B; make up the diverse nomenclature associated with the protein encoded by the RELB gene in humans. RELB is a transcription factor, also known as Transcription factor Rel B. Its name is derived from its similarity to the v-rel avian reticuloendotheliosis viral oncogene homolog B. The RELB gene plays a role in the regulation of various cellular processes, particularly in B cells. It is involved in the activation of genes that control immune responses and inflammation. Ensuring the homeostasis and proper functioning of the immune system relies on the intricate molecular pathways in which RELB participates. By rearranging the provided information, we can better understand the significance of RELB in cellular biology.

Source

Rabbit

Specificity

The Antibody for Phospho-RelB (Ser552) is designed to specifically recognize and detect the levels of endogenous RelB protein only when it is phosphorylated at the Serine 552 site. To elaborate, this antibody is capable of identifying the phosphorylated form of RelB at Ser552, indicating the activation or modulation of this particular signaling pathway. It is important to note that this antibody is not suitable for detecting non-phosphorylated RelB or any other protein variants. Its specificity towards phospho-RelB (Ser552) makes it a valuable tool for studying the phosphorylation status of RelB in various cellular processes and molecular mechanisms.

Predictive reaction species

Sheep;Bovine;Dog;Horse;

Application

The dilution ranges for different applications are as follows:
- For Western blotting (WB), the recommended dilutions range from 1:500 to 1:2000.
- For immunohistochemistry (IHC), the suggested dilutions are between 1:50 and 1:200.
- For immunofluorescence/immunocytochemistry (IF/ICC), it is recommended to use dilutions ranging from 1:100 to 1:500.
- For ELISA using peptide antigens, the optimal dilutions are between 1:20000 and 1:40000.
Please note that these dilution ranges may vary depending on the specific antibody and experimental conditions. It is advisable to perform titration experiments to determine the optimal dilution for each antibody in your experimental setup.

A peptide that has been synthesized from the human RelB protein is centered around the phosphorylation site of Serine 552. To create similar content, one could rephrase the prior statement using different words while still conveying the same meaning: A RelB-based peptide has been synthesized, focused on the Serine 552 phosphorylation site present in humans.

Properties

1mg/ml

Purification

Sequential chromatography on phospho- and non-phospho-peptide affinity columns was employed for the affinity purification of the antibody from purified rabbit serum.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to store at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the material to repeated freeze/thaw cycles as this can negatively impact its stability and quality. Therefore, it is advisable to handle the material with care and take necessary measures to minimize the risk of temperature fluctuations. Following these guidelines will help to maintain the integrity and efficacy of the stored material over an extended period of time.

Storage buffer

This product contains Rabbit IgG in a solution of phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It's recommended to store it at -20°C for best results. This product can be stored for up to 12 months from the date of receipt.

Target

Background

NF-kappa-B is a pleiotropic transcription factor found in virtually all cell types. It plays important roles in a wide range of biological processes, including inflammation, immunity, differentiation, cell growth, tumorigenesis, and apoptosis. NF-kappa-B exists as a homo- or heterodimeric complex consisting of RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL, and NFKB2/p52. These dimers bind to specific DNA sequences called kappa-B sites, with each dimer having its own unique affinity and specificity for different kappa-B sites. Depending on the dimer combination, NF-kappa-B can function as a transcriptional activator or repressor.
The activity of NF-kappa-B is tightly regulated through post-translational modifications, subcellular localization, and interactions with cofactors or corepressors. In its inactive state, NF-kappa-B complexes are sequestered in the cytoplasm by members of the NF-kappa-B inhibitor (I-kappa-B) family. Activation of NF-kappa-B involves phosphorylation of I-kappa-B by I-kappa-B kinases (IKKs) in response to various stimuli. This leads to the degradation of I-kappa-B and the release of active NF-kappa-B complexes, which then translocate to the nucleus.
Among the heterodimeric complexes, RelB-p50 and RelB-p52 act as transcriptional activators. RelB does not directly associate with DNA or interact with RELA/p65 or REL. It stimulates promoter activity in the presence of NFKB2/p49. It is also involved in the NUPR1/RELB/IER3 survival pathway, which confers resistance to cell stress in pancreatic ductal adenocarcinoma. Additionally, RelB regulates the circadian clock by suppressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer, independent of CRY1/CRY2. Notably, increased repression of the heterodimer occurs in the presence of NFKB2/p52.

Tissue specificity

Up-regulated by mitogens and NUPR1.

Celluar localization

Cytoskeleton;Cytosol;Nucleus;

Q01201