Rabbit Anti-CD3EAP Polyclonal Antibody#abs141081

Rabbit Anti-CD3EAP Polyclonal Antibody#abs141081

Please note that the price provided is for reference purposes only. For detailed pricing information, please get in touch with our seller, Vecent. It is important to remember that the quoted price may not be the final cost, so we encourage you to reach out to Vecent for further clarification....

Description

Catalog-specificationDelivery timeUSD price

abs141081-100ug

1-2 Weeks

301.0

abs141081-50ug

1-2 Weeks

201.0

Please note that the price provided is for reference purposes only. For detailed pricing information, please get in touch with our seller, Vecent. It is important to remember that the quoted price may not be the final cost, so we encourage you to reach out to Vecent for further clarification.


Overview

catalog

abs141081
Other namesERCC-1 protein has an anti-sense strand called ASE-1, which is also known as CD3-epsilon-associated protein or CD3E-associated protein. Additionally, it is referred to as PAF49 or RNA polymerase I-associated factor PAF49. Another name for ERCC-1 protein is DNA-directed RNA polymerase I subunit RPA34. People also call it CAST or MGC118851. Therefore, there are different ways of identifying ERCC-1 protein based on its associations and functions.
SourceRabbit
SpecificityThe CD3EAP Antibody is capable of detecting the total CD3EAP present in the body. This antibody specifically targets endogenous levels of CD3EAP, allowing for accurate detection and analysis.
Species ReactivityHuman
AntigenCD3EAP
ApplicationThe recommended dilution for WB is between 1:500 and 1:1000, while for IF/ICC it's 1:100 to 1:500. To carry out ELISA using a peptide, the suggested dilution is between 1:20000 and 1:40000. These dilution ranges ensure optimal performance of the assays while minimizing background signal.
ImmunogenA synthesized peptide.
MW55 KD
Properties

Concentration

1mg/ml

purificationSulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum.
ClonalityPolyclonal Antibody
Stability & StorageTo preserve the quality of the product, it is essential to store it at a temperature of -20 °C for a period of one year. It is crucial to avoid repeated freezing and thawing cycles, as this can cause damage to the product. Please ensure that the product is stored in a suitable location to maintain its integrity over time.
Storage bufferThe storage condition for Rabbit IgG is to keep it at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product has a shelf life of 12 months from the date of receipt.

Target

Background

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.

Posttranslational modificationIsoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other groups.Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
Celluar localizationCytosol;Mitochondrion;Nucleus;
UniPortO15446


Western blot analysis of extracts from A549 cells, using CD3EAP antibody.


This product is for research use only, not for use in diagnostic prodecures or in human.


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