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Rabbit Anti-CBR1 Polyclonal Antibody#abs140972

Description

Catalog-specification	Delivery time	USD price
abs140972-50ug	1-2 Weeks	201.0
abs140972-100ug	1-2 Weeks	301.0

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Overview
catalog	abs140972
Other names	Carbonyl Reductase 1, also known as NADPH-dependent carbonyl reductase 1 or CBR1, plays a crucial role in the metabolism of various compounds, including prostaglandins. It is involved in the conversion of prostaglandin E(2) to prostaglandin 9-ketoreductase and catalyzes the oxidation of 15-hydroxyprostaglandin to 15-hydroxyprostaglandin dehydrogenase. This enzyme requires NADP+ or NADPH as a cofactor in its reaction. SDR21C1 is another name for 15-hydroxyprostaglandin dehydrogenase. CBR1 is widely distributed in various tissues and is particularly prominent in the liver and kidney. It is involved in the detoxification of xenobiotics, including drugs and environmental pollutants. In addition, research studies suggest that CBR1 plays a role in the regulation of oxidative stress, inflammation, and carcinogenesis. As a highly important enzyme, any changes or mutations in CBR1 can lead to various diseases, including cancer and drug toxicity. Therefore, understanding the structure and function of CBR1 is crucial for the development of therapies and drugs to treat such diseases.
Source	Rabbit
Specificity	The detection of total CBR1 is possible with the CBR1 Antibody, which is capable of identifying endogenous levels of this protein. To reiterate, the CBR1 Antibody serves as a tool to specifically detect and measure the overall expression of CBR1 within a biological system.
Species Reactivity	Human;Mouse
Antigen	CBR1
Application	For Western Blot, the recommended dilution range is between 1:500 and 1:1000, while for immunohistochemistry, it is recommended to dilute the antibodies at 1:50 to 1:200. For the indirect immunofluorescence and immunocytochemistry techniques, the recommended dilution range is between 1:100 to 1:500. As for ELISA, when using peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to follow these dilution guidelines to ensure optimal results when using these techniques.
Immunogen	A synthesized peptide.
MW	32 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was used for peptide affinity chromatography to purify the antiserum. This purification method resulted in highly purified antiserum with specific binding capacity.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to keep it stored at a temperature of -20°C. It is advisable to avoid subjecting it to repeated freeze/thaw cycles. This storage condition should be maintained for a period of one year.
Storage buffer	The provided information states that Rabbit IgG is stored in phosphate buffered saline with a pH of 7.4. It is also mixed with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature for this solution is -20 °C. It is important to note that this solution has a shelf life of 12 months from the date it is received.
Target
Background	NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P16152