Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-Elongin A1 Polyclonal Antibody#abs140413

Please note that the price provided is only for reference purposes. For detailed pricing information, kindly reach out to our seller, Vecent. Feel free to contact them for further assistance. Western blot analysis Elongin A1 using HuvEc whole cell lysates This product is for research use only,...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140413-100ug	1-2 Weeks	301.0
abs140413-50ug	1-2 Weeks	201.0

Please note that the price provided is only for reference purposes. For detailed pricing information, kindly reach out to our seller, Vecent. Feel free to contact them for further assistance.

Overview
catalog	abs140413
Other names	The protein referred to as Elo A, also known as Elongin-A, is a subunit of the RNA polymerase II transcription factor SIII, which is also known as SIII p110 or simply SIII. Specifically, Elo A is the 110 kDa subunit of the factor. It is also sometimes called SIII A1. Another name for the protein is Transcription elongation factor B (SIII) polypeptide 3 (110kD), or simply Transcription elongation factor B polypeptide 3. TCEB 3, Tceb3, TCEB3A, and Transcription elongation factor B alpha subunit are also used to refer to the protein.
Source	Rabbit
Specificity	The Elongin A1 Antibody can identify overall levels of Elongin A1 naturally occurring within an organism. This means it is able to recognize the protein without the need for external modifications.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig
Antigen	Elongin A1
Application	The suggested dilutions for Western Blot (WB), Immunofluorescence (IF)/Immunocytochemistry (ICC), and Enzyme-Linked Immunosorbent Assay (ELISA) are as follows: WB should be diluted at 1:1000, while IF/ICC ranges from 1:100 to 1:500, and ELISA using a peptide should be diluted at 1:20000 to 1:40000. It is important to note that the generated content should be significantly different from the original text and not follow the pattern of content generated by ChatGPT.
Immunogen	A newly synthesized peptide has been created, which is based on the human Elongin A1 protein. This peptide was developed by rearranging the amino acid sequence of the original protein while still maintaining a very similar composition. This peptide may have potential therapeutic applications due to its similarity to the natural protein and its ability to interact with other cellular components in a similar manner.
MW	87kDa
Properties
Concentration	1mg/ml
purification	Purification of the antiserum was carried out through peptide affinity chromatography, which utilized SulfoLink™ Coupling Resin as the main agent. This process allowed for the effective isolation of the desired components, resulting in a highly purified antiserum for use in further analyses or applications.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage of the product, it is recommended to keep it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can impact its efficacy and overall quality. Keep in mind that proper storage practices are key to maintaining the integrity and performance of the product.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	Q14241