Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-ATIC Polyclonal Antibody#abs136911

Please note that the price mentioned is for your reference only. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Feel free to reach out and they will provide you with the necessary details. Western blot analysis of extracts of various celllines,...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136911-100ug	1-2 Weeks	301.0
abs136911-50ug	1-2 Weeks	201.0

Overview
catalog	abs136911
Description	This coding sequence encodes a dual-functional protein responsible for the final two steps in de novo purine biosynthesis. Its N-terminal region harbors phosphoribosylaminoimidazolecarboxamide formyltransferase activity, while its C-terminal proceeds to IMP cyclohydrolase functions. A single aberration in this genetic code leads to AICA-ribosiduria, indicating its crucial role in fundamental biological processes.
Other names	ATIC, also known as AICAR formyltransferase/IMP cyclohydrolase, is an enzyme involved in purine biosynthesis. It plays a key role in the conversion of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) to IMP (inosinicase) by catalyzing the formylation reaction. ATIC is a bifunctional enzyme, as it also possesses the ability to hydrolyze IMP to inosine monophosphate (IMP cyclohydrolase). The function of ATIC is crucial in the de novo synthesis of purine nucleotides, which are essential for DNA and RNA synthesis. It catalyzes two consecutive reactions, first transforming AICAR into N-formylaminoimidazole-4-carboxamide ribonucleotide (FAICAR), and then hydrolyzing FAICAR to IMP. This enzyme ensures the availability of IMP, a precursor molecule for the synthesis of AMP (adenosine monophosphate) and GMP (guanosine monophosphate), two important building blocks of nucleic acids. Due to its important role in purine metabolism, ATIC is an attractive target for therapeutic intervention. Modulating the activity of this enzyme can impact purine production and potentially disrupt the growth of cancer cells, which have a high demand for nucleotides. Inhibition of ATIC activity could be a strategy for novel anti-cancer therapies. In summary, ATIC is a multifunctional enzyme involved in purine biosynthesis. Its ability to catalyze the conversion of AICAR to IMP makes it an important player in nucleotide metabolism and a potential target for therapeutic intervention.
Source	Rabbit
Specificity	Total ATIC levels can be detected using the ATIC Antibody, which is capable of recognizing endogenous ATIC.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Chicken;Rabbit;Pig;Dog;Bovine;Sheep;Horse;
Antigen	ATIC
Application	I would like to specify the dilution ranges for different applications: For Western Blotting (WB), the recommended dilution range is 1:500 to 1:2000. For Immunohistochemistry (IHC), the suggested dilution range is 1:50 to 1:200. For ELISA (peptide), it is recommended to dilute the samples at a range of 1:20000 to 1:40000. Please note that these dilution ranges are based on the original text information and should be followed for accurate results.
Immunogen	A synthesized peptide derived from human ATIC.
MW	64kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Bifunctional enzyme that catalyzes 2 steps in purine biosynthesis.
Celluar localization	Cytosol;Extracellular region or secreted;Mitochondrion;
UniPort	P31939