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Rabbit Anti-ATF5 Polyclonal Antibody#abs133782

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Description

Catalog-specification	Delivery time	USD price
abs133782-100ug	1-2 Weeks	301.0
abs133782-50ug	1-2 Weeks	201.0

Overview
catalog	abs133782
Other names	Transcription factors play an important role in regulating gene expression. One such factor is activating transcription factor 5 (ATF5), also known as ATF7 or ATFX. This protein belongs to a family of bZIP transcription factors that bind to specific DNA sequences and activate or repress gene expression. ATF5 is involved in various cellular processes such as cell proliferation, differentiation, and apoptosis. It is also found to be overexpressed in certain cancers, making it a potential therapeutic target. Additionally, ATF5 has been shown to be involved in protein quality control and neuroprotection. The NRIF3 associated protein, also known as ODA10 or NAP1, has been identified as a transcription factor-like protein that interacts with ATF5. Its function and role in gene regulation are still under investigation. Overall, the study of ATF5 and related proteins is important for understanding cellular processes and developing new therapeutic strategies for various diseases, particularly cancer.
Source	Rabbit
Specificity	The antibody known as ATF5 Antibody is capable of detecting total ATF5 levels that occur naturally within an organism. This indicates that the antibody reacts to the expressed levels of ATF5 within an individual.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Bovine;Dog;Horse;
Antigen	ATF5
Application	WB dilution range is typically 1:500 to 1:1000, while the recommended dilution range for IF/ICC is 1:100 to 1:500. In ELISA assays, the appropriate dilution of the peptide is usually around 1:20000 to 1:40000. It is important to adhere to these dilution guidelines in order to obtain accurate and reliable results.
Immunogen	A synthesized peptide.
MW	31 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the peptide affinity chromatography process to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure its longevity, it's recommended to keep this item stored at a temperature of minus 20 degrees Celsius for up to one year. It's important to avoid subjecting the product to repeated instances of freezing and thawing, as this may cause damage. So, keeping it at the optimal temperature is vital for its preservation.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631). Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120). Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity). Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity). Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity). May be involved in osteogenic differentiation (PubMed:22442021). Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614). Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity). Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083). In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499). May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400). Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613). Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764). Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385).
Tissue specificity	Widely expressed with higher expression levels in liver.
Posttranslational modification	Ubiquitinated by CDC34 and UBE2B in order to be degraded by the proteasome. Cisplatin inhibits ubiquitination and proteasome-mediated degradation by inhibiting the interaction with CDC34 (PubMed:18458088). Ubiquitination and degradation by the proteasome are inhibited by NLK in a kinase-independent manner (PubMed:25512613).Phosphorylated by NLK, probably at Ser-92, Thr-94, Ser-126 and Ser-190.Acetylated at Lys-29 by EP300, the acetylation enhances the interaction with CEBPB, DNA-binding and transactivation activity.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	Q9Y2D1