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Rabbit Anti-FDX1 Polyclonal Antibody#abs137427

Please kindly note that the provided price is solely for your reference. For detailed pricing information, we recommend contacting our seller, Vecent. Western blot analysis FDX1 using HepG2 whole cell lysates This product is for research use only, not for use in diagnostic prodecures or in human.

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Description

Catalog-specification	Delivery time	USD price
abs137427-100ug	1-2 Weeks	301.0
abs137427-50ug	1-2 Weeks	201.0

Please kindly note that the provided price is solely for your reference. For detailed pricing information, we recommend contacting our seller, Vecent.

Overview
catalog	abs137427
Other names	Adrenal ferredoxin, also known as adrenodoxin or adrenodoxin, mitochondrial, is an essential protein involved in various mitochondrial metabolic processes. It is encoded by the ADX gene in humans and is commonly referred to as ADX or ADX_HUMAN. Other aliases for this protein include FDX, fdx1, Ferredoxin 1, Ferredoxin-1, Hepatoredoxin, LOH11CR1D, and Mitochondrial adrenodoxin. Adrenal ferredoxin plays a crucial role in steroid hormone biosynthesis by serving as an electron carrier in the mitochondrial P450 system. It transfers electrons to cytochrome P450 enzymes, which are responsible for catalyzing the conversion of cholesterol into various steroid hormones, such as cortisol, aldosterone, and sex hormones. The protein structure of adrenal ferredoxin consists of a 2Fe-2S iron-sulfur cluster that mediates electron transfer reactions. It possesses remarkable redox activity, allowing it to shuttle electrons between different enzymes involved in metabolic pathways. Mutations in the ADX gene can lead to adrenal insufficiency, a condition characterized by the impaired production of steroid hormones. This condition can have detrimental effects on various physiological processes. In summary, adrenal ferredoxin, also known as adrenodoxin or adrenodoxin, mitochondrial, is a vital protein involved in mitochondrial metabolic processes and steroid hormone biosynthesis. Its role as an electron carrier and its redox activity make it crucial for the proper functioning of various enzymatic reactions in the mitochondria.
Source	Rabbit
Specificity	The FDX1 Antibody is capable of detecting the overall levels of endogenous FDX1. To produce the equivalent content, the information from the original text has been rearranged.
Species Reactivity	Human
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	FDX1
Application	The recommended dilutions for WB are 1:1000-3000, while for IF/ICC the dilutions are 1:200-1:500. For ELISA with peptide detection, it is recommended to use dilutions ranging from 1:20000-1:40000 for optimal results. Adjusting the dilution appropriately for each application is crucial in achieving accurate and reproducible results.
Immunogen	A synthesized peptide derived from human FDX1.
MW	19 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subject to peptide affinity chromatography to purify it. It was purified to a high degree to ensure its effectiveness.
Clonality	Polyclonal Antibody
Stability & Storage	One should store the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing in order to maintain its quality.
Storage buffer	This Rabbit IgG solution comes in a phosphate buffered saline with a pH of 7.4, containing a concentration of 150mM NaCl. It also has a small fraction of sodium azide, and for preservation, 50% glycerol has been added. It is recommended to store this product at a temperature of -20 °C, where it is expected to remain stable for 12 months from the day of receipt.
Target
Background	Participates in the synthesis of thyroid hormones. Essential for the synthesis of various steroid hormones, participates in the reduction of mitochondrial cytochrome P450 for steroidogenesis. Transfers electrons from adrenodoxin reductase to CYP11A1, a cytochrome P450 that catalyzes cholesterol side-chain cleavage.
Tissue specificity	Highest levels in the adrenal gland (at protein level). Also detected in kidney and testis (at protein level).
Celluar localization	Mitochondrion;
UniPort	P10109