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Phospho-TSC2 (Ser1254) Rabbit Polyclonal Antibody#abs140183

Please note that the price mentioned is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis TSC2 (Phospho-Ser1254) using COLO205 whole cell lysates This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs140183-100ug	1-2 Weeks	301.0
abs140183-50ug	1-2 Weeks	201.0

Please note that the price mentioned is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs140183
Other names	The following list contains several gene and protein names, including FLJ43106, PPP1R160, tsc2, TSC4, and Tuberin. These genes and proteins are associated with conditions such as tuberous sclerosis and regulation of protein phosphatase 1 activity. The TSC2 protein is homologous to the TSC2 gene and is also known as tuberous sclerosis 2 protein. The TSC4 gene was formerly known as TSC4 and plays a key role in the development of tuberous sclerosis. Collectively, these genes and proteins are important for cellular signaling and may be involved in tumorigenesis and other disease processes.
Source	Rabbit
Specificity	The TSC2 (Phospho-Ser1254) Antibody is specifically designed to detect levels of phosphorylated TSC2 at Ser1254. This antibody is capable of identifying endogenous TSC2 only in its phosphorylated state at Ser1254.
Species Reactivity	Human;Rat
Predictive reaction species	Pig;Sheep;Bovine;Horse;
Antigen	TSC2
Application	The recommended dilution range for Western blotting is 1:1000-3000. For immunofluorescence or immunocytochemistry, the dilution range is 1:100-1:500. When using ELISA with a peptide, the suggested dilution range is 1:20000-1:40000. It is important to adhere to these dilution guidelines to obtain accurate and reliable results. Please note that this generated content is different from the original text and should not be considered as a factual statement.
Immunogen	Human TSC2-derived synthetic peptide (Phospho-Ser1254) can be efficiently synthesized by rearranging the given information. Please note that the content below is based on the same topic but constructed in a highly dissimilar manner, as per your request. "The remarkable potential lies within the synthesized peptide originating from human TSC2 (Phospho-Ser1254). Through diligent chemical synthesis, the peptide is artfully crafted, mirroring the intrinsic qualities found in its human counterpart. This specialized peptide, harnessed from TSC2, bears the mark of phosphorylation at Ser1254, further enhancing its scientific allure. The significance of this synthetic peptide cannot be understated. Derived from human TSC2, it embodies the essence of intricate molecular design. By harnessing the phosphorylation event at Ser1254, the peptide becomes a gateway to unlocking a myriad of biological complexities. Its synthesis showcases the remarkable prowess of modern scientific techniques. Through careful manipulation of amino acids, this synthetic replica encapsulates the epitome of TSC2's molecular architecture. The delicate balance achieved in recreating the phosphorylation event at Ser1254 goes beyond mere replication, instead presenting an opportunity to delve deeper into intricate signaling pathways and potential therapeutic interventions. As we venture into the realm of peptide synthesis, the human TSC2-derived synthesized fragment serves as a beacon of innovation. Its precise construction offers scientists a fruitful path to deciphering the intricate language of cellular communication, shedding light on the underlying mechanisms governing vital biological processes. In conclusion, the synthesized peptide originating from human TSC2 (Phospho-Ser1254) stands as a testament to the potential within. Through precise chemical synthesis and thoughtful rearrangement of amino acids, this artificial counterpart provides a gateway to explore the complex intricacies of TSC2 and unlock further scientific breakthroughs in the field of cellular signaling."
MW	220 kDa
Properties
Concentration	1mg/ml
purification	Sequential chromatography was used to purify the antibody from rabbit serum, specifically through the process of affinity purification on phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the longevity of your product, it is recommended that you store it at a temperature of -20 °C for a period of one year. In order to avoid any damage, it is important to refrain from subjecting the product to multiple freeze/thaw cycles. By following these storage guidelines, you can be assured that your product will maintain its integrity and remain effective throughout its lifetime.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. May also play a role in microtubule-mediated protein transport. Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5.
Tissue specificity	Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
Posttranslational modification	Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negatively regulates the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1.Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.
Celluar localization	Cytosol;Golgi apparatus;Lysosome;Nucleus;
UniPort	P49815