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Phospho-TIE2 (Ser1119) Rabbit Polyclonal Antibody#abs140022

Please note that the mentioned price is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent. Western blot analysis TIE2 (Phospho-Ser1119) using EGF treated 293 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs140022-100ug	1-2 Weeks	301.0
abs140022-50ug	1-2 Weeks	201.0

Please note that the mentioned price is only for your reference. For detailed pricing information, kindly reach out to our seller, Vecent.

Overview
catalog	abs140022
Other names	The Angiopoietin 1 receptor, also known as Angiopoietin-1 receptor or CD202b, is a protein involved in endothelial cell signaling. It is a tyrosine kinase receptor that is specifically expressed in endothelial cells. This receptor, encoded by the TEK gene, plays a crucial role in vascular development and maintenance. The receptor is also referred to as hTIE2, hTIE2, Hyk, p140 TEK, or TIE2 among other names. It is a transmembrane protein with Ig and EGF homology domains. The soluble variants of TIE2, known as soluble TIE2 variant 1 and soluble TIE2 variant 2, have also been identified. Mutations in the TEK gene have been associated with venous malformations multiple cutaneous and mucosal (VMCM), a rare disorder characterized by abnormal blood vessel development. The receptor is primarily expressed in endothelial cells of the tunica interna, the innermost layer of blood vessels. Understanding the functions and regulation of the Angiopoietin 1 receptor is important in elucidating the mechanisms of angiogenesis and vascular stability. It serves as a potential therapeutic target for various vascular-related diseases.
Source	Rabbit
Specificity	The TIE2 (Phospho-Ser1119) Antibody specifically recognizes and binds to TIE2 protein that is phosphorylated at Ser1119. It is designed to detect the presence of endogenous TIE2 protein only when it carries this specific phosphorylation.
Species Reactivity	Human;Mouse
Predictive reaction species	Xenopus;Zebrafish;Chicken;Pig;Sheep;Horse;Bovine;Rabbit;
Antigen	TIE2
Application	Here are three possible variations of the original content, rewritten in a different way: 1. For Western blotting, dilute the antibody at a concentration between 1:1000 and 1:3000. When performing immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500. If you plan to use the antibody for ELISA with a peptide substrate, the recommended dilution is 1:20000 to 1:40000. 2. Depending on your experimental setup, you may need to adjust the dilution of the antibody accordingly. Specifically, for Western blotting, use a dilution range of 1:1000 to 1:3000. Alternatively, if you plan to do immunofluorescence or immunocytochemistry, the recommended dilution is between 1:100 and 1:500. Finally, if you intend to use the antibody for ELISA with a peptide substrate, dilute it at 1:20000 to 1:40000. 3. To ensure optimal results with the antibody, it's essential to use the appropriate dilution for each application. For Western blotting, a dilution range of 1:1000 to 1:3000 is recommended. When performing immunofluorescence or immunocytochemistry, the antibody should be diluted at a concentration between 1:100 and 1:500. Similarly, for ELISA using a peptide substrate, dilute the antibody at 1:20000 to 1:40000.
Immunogen	A peptide synthesized from the phosphorylated serine residue at position 1119 of human TIE2 has been developed. This peptide is highly similar to the original sequence and has been designed based on the information from the original text.
MW	126KD
Properties
Concentration	1mg/ml
purification	The purified antibody was obtained from rabbit serum using affinity purification. This process involved sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.
Tissue specificity	Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
Posttranslational modification	Proteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2).Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB.Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.
Celluar localization	Cytoskeleton;Extracellular region or secreted;Plasma Membrane;
UniPort	Q02763