Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Phospho-SIK (Thr182) Rabbit Polyclonal Antibody#abs140018

Please note that the price mentioned here is for your reference only. To obtain detailed pricing information, please contact our seller, Vecent. It's important to keep in mind that any generated content should be based on the original text. Rather than using ChapGPT to generate the content, try...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs140018-100ug	1-2 Weeks	301.0
abs140018-50ug	1-2 Weeks	201.0

Overview
catalog	abs140018
Other names	There are many different names for the same protein kinase, including KID2, MSK, myocardial SNF1 like kinase, Qin-induced kinase, Salt inducible kinase 1, and Salt-inducible protein kinase 1. Additionally, it is referred to as Serine threonine protein kinase SNF1 like kinase 1, Serine threonine protein kinase SNF1LK, and Serine/threonine-protein kinase SIK1. This protein kinase is also known as Serine/threonine-protein kinase SNF1-like kinase 1, and SIK-1 or SIK1 for short. Its official gene symbol is SIK1_HUMAN. Overall, this protein kinase is important for a variety of functions in the body. It belongs to a family of enzymes that phosphorylate proteins on serine or threonine residues, which plays a role in cell signaling and regulation. Specific functions of this kinase include regulating glucose homeostasis, lipid metabolism, and gene expression. It has also been implicated in various diseases and conditions, including cancer, metabolic disorders, and inflammation. Therefore, understanding the function and regulation of this protein kinase may have important implications for human health and disease prevention.
Source	Rabbit
Specificity	The SIK (Phospho-Thr182) Antibody is designed to specifically detect the presence of phosphorylated SIK at the Thr182 site. This antibody is highly sensitive and only detects endogenous levels of SIK when it is phosphorylated at Thr182.
Species Reactivity	Human;Rat
Predictive reaction species	Chicken;Pig;Dog
Antigen	SIK
Application	The recommended dilution for Western Blot (WB) ranges from 1:1000 to 1:3000. For Immunohistochemistry (IHC), the suggested dilution is 1:50 to 1:200. If you are using ELISA with a peptide, the optimal dilution would be between 1:20000 to 1:40000. Ensure to follow these dilution guidelines for accurate and reliable results.
Immunogen	SIK（-182）。，。
MW	85KD
Properties
Concentration	1mg/ml
purification	The purification of the antibody involves sequential chromatography on affinity columns containing phospho- and non-phospho-peptides, allowing for the purification of the antibody from rabbit serum. The purified antibody is obtained from the purified rabbit serum by affinity purification.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure preservation, the recommended storage temperature for one year is -20 °C. It is crucial to avoid subjecting the product to multiple freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1 and CRTC2/TORC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1 (By similarity).
Posttranslational modification	Phosphorylated at Thr-182 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39, leading to its activation. Phosphorylation at Thr-182 promotes autophosphorylation at Ser-186, which is required for sustained activity. Autophosphorylation at Ser-186 is maintained by sequential phosphorylation at Thr-182 by GSK3-beta. GSK3-beta cannot initiate phosphorylation at Thr-182, it can only maintain it. Phosphorylation at Ser-575 by PKA promotes translocation to the cytoplasm. Phosphorylation at Thr-322 by CaMK1 following intracellular sodium concentration leads to activation.
Celluar localization	Nucleus;
UniPort	P57059