Phospho-PPARBP (Thr1032) Rabbit Polyclonal Antibody#abs140131
Please note that the price mentioned is only for your reference and if you want more specific details regarding the price, kindly get in touch with our sales representative, Vecent. You can expect to receive accurate information about pricing when you speak with Vecent. Western blot analysis...
Description
| Catalog-specification | Delivery time | USD price |
abs140131-100ug | 1-2 Weeks | 301.0 |
abs140131-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned is only for your reference and if you want more specific details regarding the price, kindly get in touch with our sales representative, Vecent. You can expect to receive accurate information about pricing when you speak with Vecent.
| Overview | |
catalog | abs140131 |
| Other names | Activator-recruited cofactor 205 kDa component, also known as ARC205 or CRSP1, is an essential component of the Mediator complex. It is involved in regulating transcription by RNA polymerase II and plays a significant role in various cellular processes. This cofactor, also known as DRIP205 or DRIP230, is crucial for the recruitment of activators to the transcription initiation complex. It interacts with a variety of transcription factors, including p53, and acts as a regulatory protein (RB18A). Furthermore, this component is a PPAR-binding protein (PPARBP) and a component of the vitamin D receptor-interacting protein complex. It is also involved in thyroid hormone receptor-associated protein complex formation, where it is referred to as Trap220 or TRIP-2. The Mediator complex subunit 1, or MED1, is the human homolog of the yeast Mediator subunit Med1/Srb4. It plays a vital role in transducing signals from transcription factors to the RNA polymerase II machinery, thereby facilitating gene expression. In conclusion, Activator-recruited cofactor 205 kDa component, with its various aliases and interactions, plays a central role in mediating transcriptional activation and regulating key cellular processes. |
| Source | Rabbit |
| Specificity | The PPARBP (Phospho-Thr1032) Antibody is designed to specifically detect the presence of phosphorylated PPARBP at Thr1032 in endogenous levels. This means that the antibody is highly selective and will only recognize the protein when it is in its phosphorylated state at this specific site. Other forms or modifications of PPARBP will not be detected by this antibody. |
| Species Reactivity | Human;Mouse |
| Predictive reaction species | Chicken;Rabbit;Sheep;Dog;Bovine;Horse; |
| Antigen | PPARBP |
| Application | Here are some possible options: 1. When it comes to Western blotting, we recommend diluting your primary antibody at a ratio of 1:1000-3000. For immunofluorescence and immunocytochemistry, a dilution of 1:100-1:500 should work well. If you're using ELISA with a peptide substrate, we suggest a dilution of 1:20000-1:40000 for your antibody. 2. If you're planning to do some Western blotting, we'd suggest diluting your antibody at a rate of 1:1000-3000. For immunofluorescence and immunocytochemistry, a dilution of 1:100-1:500 is recommended. And if you're using ELISA with a peptide antigen, you'll likely need a dilution of 1:20000-1:40000 for your antibody. 3. To get the best results with Western blotting, we recommend diluting your primary antibody at a ratio of 1:1000-3000. If you're doing immunofluorescence or immunocytochemistry, a dilution of 1:100-1:500 will likely be appropriate. And if you're using ELISA with a peptide substrate, we suggest a dilution of 1:20000-1:40000 for the antibody you choose. 4. If you're performing Western blotting, we would suggest diluting your primary antibody at a rate of 1:1000-3000. For immunofluorescence or immunocytochemistry, dilution at 1:100-1:500 should work well. For ELISA with a peptide antigen, we suggest a dilution of 1:20000-1:40000 for your chosen antibody. 5. For optimal results in Western blotting, we recommend diluting your primary antibody at 1:1000-3000. When conducting immunofluorescence or immunocytochemistry, you should dilute it to 1:100-1:500. As for ELISA with a peptide substrate, a dilution of 1:20000-1:40000 for the antibody should suffice. |
| Immunogen | PPARBP (Phospho-Thr1032) is the origin of a synthesized peptide that exhibits high similarity to the content mentioned above. The peptide is derived from human PPARBP and shows phosphorylation on Thr1032. It is crucial to emphasize that the content generated should be distinct from the typical conversational style of ChapGPT. Instead, let's explore a novel approach using the language model to deliver the information. |
| MW | 168kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The rabbit serum was subjected to affinity purification using both phospho- and non-phospho-peptide affinity columns in order to obtain the purified antibody. This sequential chromatography process resulted in a highly specific and pure antibody. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
| Tissue specificity | Ubiquitously expressed. |
Western blot analysis PPARBP (Phospho-Thr1032) using Na3VO4 treated K562 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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