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Collagen I Antibody#abs131984

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Description

Catalog-specification	Delivery time	USD price
abs131984-50ug	In Stock	201.0
abs131984-100ug	In Stock	301.0

Overview
catalog	abs131984
Description	This particular gene is responsible for producing one of the chains necessary for the formation of type I collagen, which is a crucial component of connective tissues in the body. Numerous genetic mutations in this gene have been linked to various conditions including osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. However, the impact of these gene mutations tends to be milder compared to mutations in the gene responsible for alpha-1 type I collagen, mainly because alpha-2 collagen is present in smaller quantities. Hence, individuals with mutations in this gene may experience less severe symptoms.
Other names	The alpha 1 type I collagen and alpha 2 type I collagen are key components of skin, tendons and bones. They are encoded by the COL1A1 and COL1A2 genes, respectively. The alpha 2 type I procollagen, also known as alpha 2(I) procollagen or alpha 2(I)-collagen, is a precursor protein that is cleaved to form the mature collagen molecules. The alpha1(I) procollagen or pro-alpha-1 collagen type 1 is another precursor protein of collagen type I. It is encoded by the COL1A1 gene and is essential for the formation of the collagen triple helix structure. The collagen of skin, tendon and bone, alpha-1 chain and collagen of skin, tendon and bone, alpha-2 chain form the alpha chains of type I collagen. These chains are interwoven in a triple helix structure to form mature type I collagen molecules. Mutations in these genes can cause osteogenesis imperfecta (OI), a genetic disorder that affects bone strength and causes brittle bones. The collagen type I alpha 1 and alpha 2 polypeptides are responsible for the tensile strength of bones, skin, and tendons due to their high resistance to tension. These collagens play critical roles in extracellular matrix (ECM) formation and organization, providing a scaffold for cell migration and tissue repair. In summary, type I collagen is an important component of skin, tendon, and bone extracellular matrix providing the strength and resilience required for various biological processes.
Source	Rabbit
Specificity	The antibody for Collagen I is designed to specifically detect the levels of total endogenous Collagen I in samples. By utilizing this antibody, researchers can accurately measure the presence of Collagen I in various biological samples.
Species Reactivity	Human;Mouse;Rat;Bovine
Predictive reaction species	Pig;Bovine;Dog;Horse;
Antigen	Collagen I
Application	In the recommended dilution range for immunohistochemistry (IHC), the ratio is typically between 1:50 and 1:200. For immunofluorescence/immunocytochemistry (IF/ICC), the ratio is commonly seen at 1:100 to 1:500. When it comes to enzyme-linked immunosorbent assay (ELISA), the dilution is usually around 1:20000 to 1:40000 for general detection, while for ELISA using peptides, it remains the same at 1:20000 to 1:40000.
Immunogen	A peptide has been synthesized that is derived from Collagen I in humans. To create a similar content, one could rephrase the original text information to convey the same idea.
MW	130-150 kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Tissue specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Posttranslational modification	Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;
UniPort	P08123

abs131984 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

abs131984 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Western blot analysis of extracts of various samples,using Collagen I Antibody.

Immunohischemistry analysis of paraffin-embedded human breast carcinoma tissue, using Collagen I antibody.

Western blot analysis of Collagen I expression in Mouse Brain lysates,The lane on the left is treated with the antigen-specific peptide.