Phospho-FGFR1/2/3/4 (Tyr653+Tyr654) Rabbit Polyclonal Antibody#abs140039
Please note that the price mentioned above is for reference only. If you require detailed pricing information, we kindly ask you to get in touch with our sales representative, Vecent. Western blot analysis FGFR1/2/3/4 (Phospho-Tyr653+Tyr654) using Serum treated COS7 whole cell lysates This...
Description
| Catalog-specification | Delivery time | USD price |
abs140039-100ug | 1-2 Weeks | 301.0 |
abs140039-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is for reference only. If you require detailed pricing information, we kindly ask you to get in touch with our sales representative, Vecent.
| Overview | |
catalog | abs140039 |
| Other names | The fibroblast growth factor receptors (FGFRs) are a group of proteins that play a crucial role in cell growth, development, and wound healing. They are involved in various cellular processes, including proliferation, differentiation, and angiogenesis. FGFRs are transmembrane tyrosine kinase receptors that bind to fibroblast growth factors (FGFs) and activate intracellular signaling pathways. There are four known types of FGFRs in humans: FGFR1, FGFR2, FGFR3, and FGFR4. Each type has multiple isoforms generated by alternative splicing. FGFR1, also known as basic fibroblast growth factor receptor 1 (bFGF-R-1), is a receptor for the fibroblast growth factor 2 (FGF2), or basic FGF. It is expressed in various tissues and cell types, including neurons, endothelial cells, fibroblasts, osteoblasts, and smooth muscle cells. FGFR2 is involved in the development of many organs and tissues, including the skeleton, limbs, heart, and nervous system. Mutations in the FGFR2 gene have been associated with various craniofacial disorders, such as Jackson Weiss syndrome and Apert syndrome. FGFR3 is primarily expressed in bone and cartilage tissues and plays a critical role in skeletal development. Mutations in the FGFR3 gene are associated with achondroplasia, a common form of dwarfism, as well as other skeletal dysplasias. FGFR4 is widely expressed in various tissues and is involved in liver, lung, and breast cancer development and progression. It has been shown to regulate cell proliferation, survival, and migration. Overall, the fibroblast growth factor receptors are essential for normal development and tissue homeostasis. Dysregulation of FGFR signaling can lead to various developmental disorders and diseases, making them potential targets for therapeutic interventions. |
| Source | Rabbit |
| Specificity | This antibody is specifically designed to detect the presence of phosphorylated FGFR1/2/3/4 at Tyr653+Tyr654 residues, while demonstrating no cross-reactivity with their non-phosphorylated forms. It is sensitive enough to identify endogenous levels of FGFR1/2/3/4 in biological samples, making it a valuable tool for research studies focusing on the study of tyrosine kinase signaling pathways. By targeting this specific phosphorylation site, researchers can gain a better understanding of the role that FGFR1/2/3/4 plays in physiological and pathological conditions. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Chicken;Rabbit;Xenopus;Dog;Horse;Sheep;Bovine |
| Antigen | FGFR1/2/3/4 |
| Application | ： ，。（WB），1:10001:3000。（IHC）1:501:200。ELISA（），1:200001:40000。 ，。 |
| Immunogen | A synthesized peptide derived from human FGFR1/2/3/4 (Phospho-Tyr653+Tyr654). |
| MW | 92KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation. |
| Tissue specificity | Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. |
| Posttranslational modification | Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer and proceeds in a highly ordered manner. Initial autophosphorylation at Tyr-653 increases the kinase activity by a factor of 50 to 100. After this, Tyr-583 becomes phosphorylated, followed by phosphorylation of Tyr-463, Tyr-766, Tyr-583 and Tyr-585. In a third stage, Tyr-654 is autophosphorylated, resulting in a further tenfold increase of kinase activity. Phosphotyrosine residues provide docking sites for interacting proteins and so are crucial for FGFR1 function and its regulation.Ubiquitinated. FGFR1 is rapidly ubiquitinated by NEDD4 after autophosphorylation, leading to internalization and lysosomal degradation. CBL is recruited to activated FGFR1 via FRS2 and GRB2, and mediates ubiquitination and subsequent degradation of FGFR1.N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. |
| Celluar localization | Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane; |
| UniPort | P11362/P21802/P22607/P22455 |
Western blot analysis FGFR1/2/3/4 (Phospho-Tyr653+Tyr654) using Serum treated COS7 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
Hot Tags: phospho-fgfr1/2/3/4 (tyr653+tyr654) rabbit polyclonal antibody#abs140039, China phospho-fgfr1/2/3/4 (tyr653+tyr654) rabbit polyclonal antibody#abs140039 suppliers
Send Inquiry
You Might Also Like
