Phospho-c-Kit (Tyr721) Rabbit Polyclonal Antibody#abs130781

abs130781-50ug

1-2 Weeks

201

abs130781-100ug

1-2 Weeks

301

Description

The human homolog of the proto-oncogene c-kit is encoded by KIT, which was initially discovered as the cellular equivalent of the feline sarcoma viral oncogene v-kit. MGF or stem cell factor binds to KIT, which functions as a type 3 transmembrane receptor. Various disorders, including gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism, are linked to mutations in KIT.

Other names

The gene known as c-Kit, or CD117, encodes for a receptor tyrosine kinase called KIT. Also referred to as Kit or stem cell factor receptor (SCFR), this protein is involved in various biological processes. The ligand for c-Kit, known as c-Kit Ligand or Kit Ligand, regulates its activity. Some other aliases for c-Kit include KIT oncogene, Mast cell growth factor receptor, Mast/stem cell growth factor receptor Kit, MGF, p145 c-kit, PBT, Piebald trait protein, Proto oncogene c Kit, Proto oncogene tyrosine protein kinase Kit, Proto-oncogene c-Kit, soluble KIT variant 1, Steel Factor Receptor, and Tyrosine-protein kinase Kit. In felines, the homolog of the v-kit Hardy Zuckerman 4 feline sarcoma viral oncogene plays a similar role. This information can be rearranged and combined to create a highly similar content, maintaining the core details of the original text.

Source

Rabbit

Specificity

The Phospho-c-Kit (Tyr721) Antibody is designed to specifically recognize and bind to c-Kit protein that has been phosphorylated at Tyrosine 721. This antibody is highly specific in its detection of endogenous levels of c-Kit and will not detect the protein if it is not phosphorylated on this specific site. By detecting the phosphorylation state of c-Kit, researchers can gain insights into the signaling pathways and biological processes influenced by this important protein.

Predictive reaction species

Rabbit;Pig;Dog;Horse;Bovine

Application

Please note that the following content is based on the provided information but has been rearranged to generate a highly similar version:
The recommended dilutions for Western blot (WB) range between 1:500 and 1:2000. For immunohistochemistry (IHC), dilutions of 1:100 to 1:500 are suggested. When performing immunofluorescence/immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 can be used. In ELISA assays using peptides, a dilution range of 1:20000 to 1:40000 is recommended. Please follow these guidelines to ensure accurate and reliable results.

A peptide that has been synthesized from the region of human c-Kit centered on the phosphorylation site of Tyrosine 721 is now available. This newly created peptide is designed to closely resemble the original sequence and be highly effective as a research tool. Researchers can now use this peptide in a variety of experiments to study the function of c-Kit and its phosphorylation at Tyrosine 721. This exciting discovery will undoubtedly lead to new insights into the behavior of c-Kit and its role in human biology and disease.

Properties

1mg/ml

Purification

The method used to obtain this antibody involved purifying rabbit serum and using sequential chromatography on affinity columns for both phospho- and non-phospho-peptides. The resulting antibody is highly specific and effective in its target binding. This process ensures a high degree of purity and yields an antibody that is suitable for various downstream applications. Overall, this methodology is a reliable and efficient way to obtain high-quality antibodies with excellent specificity and sensitivity.

Clonality

Polyclonal Antibody

To preserve the quality of the product, it is essential to store it at a temperature of -20 °C for a period of one year. Make sure to avoid exposing it to repeated cycles of freezing and thawing. This will help to ensure that the product remains stable and effective, as intended.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Tissue specificity

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Celluar localization

Extracellular region or secreted;Lysosome;Plasma Membrane;

P10721