Phospho-KIT (Tyr703) Rabbit Polyclonal Antibody#abs130780

abs130780-50ug

1-2 Weeks

201

abs1307180-100ug

1-2 Weeks

301

Description

The human homolog of the proto-oncogene c-kit is encoded by KIT. It was first recognized as the cellular counterpart to the feline sarcoma viral oncogene v-kit. KIT functions as a transmembrane receptor of type 3 and interacts with MGF (mast cell growth factor), also referred to as stem cell factor. Genetic alterations in KIT have been linked to various medical conditions such as gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. To create a highly similar content, the original information was rephrased and rearranged accordingly.

Other names

The c-Kit protein, also known as CD117 or KIT proto-oncogene receptor tyrosine kinase, plays an important role in cell proliferation, differentiation, and survival in various cell types. The c-Kit protein is encoded by the KIT oncogene and is activated by its ligand, stem cell factor (SCF). The SCF receptor (SCFR) is also known as the mast/stem cell growth factor receptor Kit. Mutations in the c-Kit gene have been associated with several human diseases, including gastrointestinal stromal tumors (GISTs) and mastocytosis. The c-Kit protein has been targeted by various drugs, including tyrosine kinase inhibitors, for the treatment of these diseases. Despite its importance, the mechanism of c-Kit signaling is still not fully understood, and further research is needed to fully elucidate its role in cellular processes.

Source

Rabbit

Specificity

The Phospho-KIT (Tyr703) Antibody is specifically designed to target KIT when it is phosphorylated at Tyrosine 703, and can detect only endogenous levels of this protein. This antibody is valuable for research purposes as it helps researchers determine the levels of a specific protein under certain conditions. By detecting the phosphorylation of KIT at Tyrosine 703, this antibody can help researchers gain insights into the signaling pathways that regulate this protein and its role in various biological processes.

Predictive reaction species

Pig;Horse;

Application

To use this antibody effectively, we recommend dilutions of WB 1:500-1:2000, IHC 1:50-1:200, IF 1:200, and ELISA (peptide) 1:20000-1:40000. These are optimal conditions for producing accurate and consistent results in your experiments. By taking these dilution factors into account, you can ensure that your antibody is used in the most effective way possible and that you get the most reliable data possible. So don't hesitate to use this antibody in your next experiment!

Human KIT, specifically the region encompassing Tyrosine 703 phosphorylation site, serves as the basis for the development of a chemically produced peptide. Through rearrangement of the aforementioned information, a comparable content can be constructed while maintaining the essence of the original text. Please note that the approach for generating the content should deviate from the manner of conversation employed by ChapGPT, instead focusing on producing text that is markedly distinct.

Properties

1mg/ml

Purification

The antibody was obtained via an affinity purification process utilizing both phospho- and non-phospho-peptide affinity columns. Purified rabbit serum was used as the source material for this process. The resulting antibody is highly specific and suited for various applications in research and diagnostics.

Clonality

Polyclonal Antibody

To maintain the quality of the product, it is recommended to store it at a temperature below -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this can affect its efficacy. Therefore, proper storage and handling of the product should be maintained at all times to ensure its effectiveness.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Tissue specificity

Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

Celluar localization

Extracellular region or secreted;Lysosome;Plasma Membrane;

P10721