Phospho-DRP-2 (Thr514) Rabbit Polyclonal Antibody#abs131084

abs131084-50ug

1-2 Weeks

201

abs131084-100ug

1-2 Weeks

301

Description

The enzyme CRMP-2 functions as a dihydropyrimidinase and is involved in RhoA-mediated signaling. It interacts with and regulates Rho kinase, contributing to neurogenesis. However, in fetal Down syndrome brain, CRMP-2 expression is abnormal.

Other names

Dihydropyrimidinase-related protein 2, also known as CRMP-2 or ULIP-2, is a collapsin response mediator protein that plays an important role in neuronal function. It is involved in axon guidance, dendrite formation, and synaptic plasticity. Its function is regulated by phosphorylation, and dysregulation of its phosphorylation has been linked to neurological disorders such as Alzheimer's disease and epilepsy.
CRMP-2 is a dihydropyrimidinase-like protein that is expressed in the nervous system. It is also found in other tissues such as muscle and lung. It is involved in the regulation of the cytoskeleton, which is important for cell shape and movement. It interacts with microtubules and actin filaments to regulate the stability and dynamics of these structures.
CRMP-2 has been shown to interact with a number of other proteins, including Numb, MAGUK, and Munc18. These interactions are important for its function in the nervous system. It also interacts with a number of neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor, which is involved in learning and memory.
In summary, CRMP-2 is an important protein involved in the regulation of neuronal function. Its dysregulation has been linked to a number of neurological disorders. Understanding its function and regulation is important for the development of therapies for these disorders.

Source

Rabbit

Specificity

The Phospho-DRP-2 (Thr514) Antibody specifically recognizes the phosphorylation of DRP-2 at Threonine 514. This antibody is designed to detect the endogenous levels of DRP-2 only when it is phosphorylated at this specific site.

Predictive reaction species

Chicken;Rabbit;Pig;Dog;Sheep;Horse;Bovine

Application

The recommended dilution ranges for WB, IHC, IF/ICC, and ELISA(peptide) are as follows:
- WB: 1:500-1:2000
- IHC: 1:50-1:200
- IF/ICC: 1:100-1:500
- ELISA(peptide): 1:20000-1:40000.
To achieve optimal results, it is advisable to dilute the samples within these dilution ranges.

The phosphorylation site of Threonine 514 serves as the basis for a synthesized peptide derived from human DRP-2. We need to rearrange the original content to generate a highly similar piece of information. Please note that this should be done without following the conversation style of ChapGPT and instead presenting the information in a completely different manner.

Properties

1mg/ml

Purification

The antibody was obtained through a purification process involving affinity purification on phospho- and non-phospho-peptide affinity columns using purified rabbit serum. This sequential chromatography method was employed to ensure the high specificity and purity of the antibody.

Clonality

Polyclonal Antibody

To maintain its potency, it is recommended to keep the product in a freezer at -20 °C for a year. It is essential to avoid exposing the substance to multiple freezing and thawing cycles, as it could diminish its efficacy.

Storage buffer

Please note the following information regarding the storage and stability of Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol:
- The product should be stored at -20 °C.
- It is stable for a duration of 12 months from the date of receipt.

Target

Background

 Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. May play a role in endocytosis.

Tissue specificity

Ubiquitous.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Plasma Membrane;

Q16555