Rabbit Anti-Nrf2 Polyclonal Antibody #abs130481

abs130481-50ug

In Stock

201

abs130481-100ug

In Stock

301

Description

The transcription factor GABPA plays a crucial role in the activation of the Adenovirus E4 gene by its ability to interact with GA repeats, which are purine-rich DNA sequences. GABPA, a member of the ETS family, forms a heterotetramer composed of two alpha and two beta subunits. Its presence is essential for the proper expression of the E4 gene. This information has been rearranged to provide a highly similar yet distinct content.

Other names

NF-E2-related factor 2 (Nrf2), also known as nuclear factor erythroid 2-like 2 (NFE2L2), is a protein involved in the regulation of gene expression. It is derived from erythroid cells and plays a crucial role in cellular defense against oxidative stress.
Nrf2 acts as a transcription factor, binding to DNA and activating the expression of antioxidant and detoxification genes. It is also responsible for maintaining cellular homeostasis by regulating the balance between oxidative stress and antioxidant defense.
The function of Nrf2 is mediated through its interaction with various regulatory proteins, including HEBP1 (heparin-binding EGF-like growth factor 1), NF E2 related factor 2, and NF2L2_HUMAN. These interactions modulate the activity of Nrf2 and regulate its ability to activate target genes.
The importance of Nrf2 in cellular protection is highlighted by its association with various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. Dysregulation of Nrf2 activity can lead to increased susceptibility to oxidative damage and impaired cellular function.
Understanding the molecular mechanisms that regulate Nrf2 activity has significant implications for the development of therapeutic interventions targeting oxidative stress-related diseases. Further research is needed to elucidate the intricate regulatory networks involving Nrf2 and its interacting partners and to explore its therapeutic potential.

Source

Rabbit

Specificity

Nrf2 Antibody detects endogenous levels of Nrf2.

Species Reactivity

Human;Mouse;Rat

Application

The dilutions for Western blot (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the dilutions are between 1:50 to 1:200. As for immunofluorescence/immunocytochemistry (IF/ICC), the dilutions range from 1:100 to 1:500. When performing ELISA with peptides, the dilutions are typically between 1:20000 to 1:40000. It is important to note that this generated content is based on the original text information, but presented in a different format.

Immunogen

A synthesized peptide derived from human Nrf2.

Properties

Concentration

1mg/ml

Purification

To purify the antiserum, the SulfoLink™ Coupling Resin was employed in peptide affinity chromatography.

Clonality

Polyclonal Antibody

Stability & Storage

To maintain the quality and effectiveness of the product, it is recommended to store it at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this can compromise its stability and efficacy. By following these guidelines, you can ensure that the product remains viable and delivers optimal results.

Storage buffer

This product is Rabbit IgG dissolved in a solution of 150mM NaCl, pH 7.4 phosphate buffered saline, with 0.02% sodium azide and 50% glycerol. It is recommended to store this product at -20 °C and it should remain stable for up to a year from the date of receipt.

Target

Background

A transcription activator is a protein that specifically binds to antioxidant response (ARE) elements found in the promoter regions of target genes. Its role is crucial in coordinating the up-regulation of genes in response to oxidative stress. Remarkably, this activator may also play a significant part in the transcriptional activation of genes within the beta-globin cluster. By mediating the enhancer activity of hypersensitive site 2 in the beta-globin locus control region, it contributes to the regulation of gene expression in this critical genetic region. Through its binding and activation, this transcription activator ensures the proper response to oxidative stress and maintains the proper functioning of genes within the beta-globin cluster.

Tissue specificity

The expression of this gene is widely observed, with the highest levels found in adult muscle, kidney, lung, liver, and fetal muscle. The gene is found to be expressed in various tissues, but reaches its peak in adult muscle, kidney, lung, liver, and fetal muscle.

Posttranslational modification

Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.Acetylation at Lys-596 and Lys-599 increases nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence.Ubiquitinated by the KEAP1-CUL3-RBX1 E3 ubiquitin ligase complex and subject to proteasomal degradation. Ubiquitination is inhibited by sulforaphane.

Celluar localization

Cytoskeleton;Cytosol;Golgi apparatus;Nucleus;Plasma Membrane;

UniPort

Q16236