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Rabbit Anti-CASP9 Polyclonal Antibody#abs136568

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts of various celllines, using CASP9antibody. This product is for research use only, not for use...

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Description

Catalog-specification	Delivery time	USD price
abs136568-100ug	1-2 Weeks	301.0
abs136568-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs136568
Description	Caspase-9, also known as ICE-LAP6 or Mch6, is a significant member of the caspase family, which consists of cysteine aspartic acid proteases. It plays a critical role in apoptosis (1,2). When cells undergo apoptotic stimulation, cytochrome c is released from the mitochondria and combines with the 47 kDa procaspase-9/Apaf 1. This leads to the activation of caspase-9 mediated by Apaf-1 (3-6). During this process, caspase-9 undergoes intrinsic proteolytic processing, resulting in cleavage at Asp315. This produces a p35 subunit. Additionally, another cleavage occurs at Asp330, generating a p37 subunit. The presence of the p37 subunit can enhance the apoptotic response, amplifying the overall effect (3-6). Subsequently, the cleaved caspase-9 further processes other caspase members such as caspase-3 and caspase-7. This initiates a caspase cascade, ultimately leading to apoptosis (7-10).
Other names	Caspase 9 is also known as APAF-3, apoptotic protease Mch-6, and ICE-LAP6. It is an apoptosis-related cysteine peptidase and a key player in the apoptotic pathway. Caspase 9 is activated through its interaction with apoptotic protease-activating factor 3 (APAF3), which forms the apoptosome complex. Once activated, caspase 9 cleaves and activates downstream effector caspases, leading to cell death. Additionally, caspase 9 has been shown to play a role in a variety of biological processes beyond apoptosis, including inflammation and immunity. It is regulated by various mechanisms, including phosphorylation and interaction with other proteins, such as protein phosphatase 1, regulatory subunit 56 (PPP1R56). Caspase 9 has also been studied as a potential therapeutic target, with caspase 9 inhibitors being developed for use in cancer treatment. Moreover, dominant-negative mutants of caspase 9 have been generated to investigate the role of caspase 9 in various disease models. Overall, caspase 9 is a complex and multifaceted protein involved in many aspects of cell physiology and pathology.
Source	Rabbit
Specificity	The endogenous levels of total CASP9 can be detected using the CASP9 Antibody. It is designed to specifically target and measure the total CASP9 protein present in a biological sample. By detecting and quantifying the levels of CASP9, the antibody provides valuable information about its expression and potential biological functions.
Species Reactivity	Human;Mouse;Rat
Antigen	CASP9
Application	：WB（）1:500-1:2000，IF/ICC（/）1:100-1:500，ELISA（，）1:20000-1:40000。，。
Immunogen	A synthesized peptide derived from human CASP9.
MW	46kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Tissue specificity	Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.
Posttranslational modification	Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.
Celluar localization	Cytosol;Mitochondrion;Nucleus;
UniPort	P55211