Phospho-CaMK4 (Thr196/200) Rabbit Polyclonal Antibody#abs131085

abs131085-50ug

1-2 Weeks

201

abs131085-100ug

1-2 Weeks

301

Description

Belonging to the serine/threonine protein kinase family and the Ca(2+)/calmodulin-dependent protein kinase subfamily, this gene's product is a multifunctional kinase with limited expression in tissues. Its involvement in transcriptional regulation has been observed in lymphocytes, neurons, and male germ cells.

Other names

Type 4 brain calcium/calmodulin-dependent protein kinase, also known as CAM kinase 4 or CaMK4, is a crucial enzyme involved in signal transduction pathways. Its activation is mediated by calcium and calmodulin, leading to phosphorylation of various protein targets. CaMK4 plays a significant role in neuronal development, synaptic plasticity, and gene expression regulation. Furthermore, it is implicated in learning and memory processes. The catalytic chain of CaMK4 is responsible for its enzymatic activity, and it is specifically activated by calcium and calmodulin binding. The significance of CaMK4 in cellular signaling has been widely studied, and it holds promise as a potential therapeutic target for neurological disorders.

Source

Rabbit

Specificity

The antibody for Phospho-CaMK4 (Thr196/200) specifically recognizes CaMK4 when it is phosphorylated at Threonine 196/200. This antibody is capable of detecting endogenous levels of phosphorylated CaMK4. To generate similar content in a different format, we could say:
"The Phospho-CaMK4 (Thr196/200) Antibody is designed to selectively identify and measure the presence of CaMK4 in its phosphorylated state at Threonine 196/200. It is effective in detecting the naturally occurring levels of phosphorylated CaMK4 within cells or tissues."

Predictive reaction species

Xenopus;Zebrafish;Chicken;Pig;Dog;Horse;Sheep

Application

For optimal results, we recommend using our antibody at a dilution range of 1:500 to 1:2000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, 1:100 to 1:500 for immunofluorescence and immunocytochemistry, and 1:20000 to 1:40000 for ELISA (peptide). Please note that the dilution range may vary depending on the sample type and preparation, so we suggest performing a titration experiment to determine the optimal dilution for your particular application.

One potential variation of the original content could be:
A peptide synthesized from a specific region of human CaMK4, centered around the Threonine 196/200 phosphorylation site. This synthesized peptide closely mimics the structure and function of the original protein sequence, providing a valuable tool for studying kinase activity and potential drug targets. By manipulating the peptide's structure and testing its effects on cellular signaling pathways, researchers can gain insights into the complex regulatory mechanisms of CaMK4 and its interactions with other proteins. Ultimately, this knowledge may lead to new therapeutic approaches for treating diseases related to CaMK4 dysfunction.

Properties

1mg/ml

Purification

The purified rabbit serum was used to extract the antibody through affinity purification by passing it through both phospho- and non-phospho-peptide affinity columns in a sequential order. This process was carried out to ensure the highest quality and purity of the antibody.

Clonality

Polyclonal Antibody

To preserve the integrity of the product, it is recommended to keep it at a temperature of -20 °C for one year without subjecting it to repeated freeze/thaw cycles. It is crucial to avoid any fluctuations in temperature to ensure its stability.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4+/CD8+ double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2). Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs). Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

Tissue specificity

Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.

Celluar localization

Cytosol;Extracellular region or secreted;Nucleus;

Q16566