Phospho-p21 Cip1 (Thr145) Rabbit Polyclonal Antibody#abs130917

abs130917-50ug

1-2 Weeks

201

abs130917-100ug

1-2 Weeks

301

Description

This potent cyclin-dependent kinase inhibitor is encoded by a specific gene. Its primary function is to bind to and inhibit the activity of cyclin-CDK2 or -CDK4 complexes, which in turn regulates the progression of the cell cycle at the G1 phase. The expression of this gene is under strict control by the tumor suppressor protein p53, allowing the protein to play a crucial role in mediating the p53-dependent cell cycle G1 phase arrest. This response is triggered in the presence of various stress stimuli.

Other names

CDK-interacting protein 1, also known as CDKI and Cyclin-dependent kinase inhibitor 1A (P21), is a protein that plays a crucial role in regulating cell growth and division. It is encoded by the CDKN1A gene and is also known by several other names such as p21, P21 protein, and Wild type p53 activated fragment 1 (WAF1).
This protein acts as a DNA synthesis inhibitor and functions by binding to and inhibiting the activity of cyclin-dependent kinases (CDKs), which are enzymes responsible for promoting cell division. CDK-interacting protein 1 is therefore considered an important tumor suppressor as it helps prevent uncontrolled cell growth and the development of cancer.
Other aliases of this protein include MDA-6, SDI1, PIC1, and SLC12A9. It is also known as Melanoma differentiation-associated protein 6, reflecting its role in promoting differentiation in melanoma cells.
In conclusion, CDK-interacting protein 1 is a vital protein that plays a crucial role in regulating cell growth and division. It acts as a DNA synthesis inhibitor and helps prevent uncontrolled cell growth, making it an important tumor suppressor.

Source

Rabbit

Specificity

The Phospho-p21 Cip1 (Thr145) Antibody is able to specifically detect p21 Cip1 when it is phosphorylated at threonine 145. This antibody detects endogenous levels of the protein, indicating its utility for studying natural biological processes involving p21 Cip1. By recognizing the phosphorylated form of p21 Cip1, this antibody can provide valuable insight into the regulation of cell cycle progression and cell proliferation. Researchers can use this antibody to investigate the functional significance of phosphorylation at threonine 145 and its potential involvement in disease processes. Overall, the Phospho-p21 Cip1 (Thr145) Antibody is a valuable tool for the study of p21 Cip1 biology and its potential role in various cellular processes.

Predictive reaction species

Sheep;Bovine;Dog

Application

The dilutions for WB (western blotting) range from 1:500 to 1:2000. For IHC (immunohistochemistry), the dilutions are between 1:50 and 1:200. IF/ICC (immunofluorescence/immunocytochemistry) requires dilutions ranging from 1:100 to 1:500. Lastly, for ELISA (enzyme-linked immunosorbent assay) using peptides, the dilutions are from 1:20000 to 1:40000. Please note that the content has been rearranged to provide the same information in a different order.

A peptide synthesized from human p21 Cip1 that focuses on Threonine 145 phosphorylation site has been created. The resulting peptide is highly similar to the original sequence and maintains its uniqueness. The aim of this synthesis is to study the effects of specific phosphorylation on p21 Cip1 and its role in cell cycle regulation. The peptide can be used for further research on this important protein and its functions in various cellular processes.

Properties

1mg/ml

Purification

The purified rabbit serum yielded the antibody via a process of sequential chromatography involving phospho- and non-phospho-peptide affinity columns for affinity purification. An antibody was obtained from this process that was highly similar to the original serum.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to keep the product in a freezer set at a temperature of -20 °C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may adversely affect its quality and efficacy. Therefore, it is advised to handle the product with care and avoid unnecessary exposure to temperature fluctuations. By following these guidelines, you can ensure that the product remains stable and effective over an extended period of time.

Storage buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

 May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).

Tissue specificity

Expressed in all adult tissues, with 5-fold lower levels observed in the brain.

Celluar localization

Cytosol;Nucleus;

P38936