Phospho-STAT3 (Tyr705) Rabbit Polyclonal Antibody#abs130918

abs130918-50ug

1-2 Weeks

201

abs130918-100ug

1-2 Weeks

301

Description

The gene in question codes for a type of protein that belongs to the STAT family. These proteins play a vital role in transcriptional activation, by forming homo- or heterodimers, which are capable of transporting themselves to the cell nucleus. Upon exposure to cytokines or other growth factors, receptor-associated kinases phosphorylate STAT members, and consequently, they carry out their transcriptional activation.

Other names

The gene 1110034C02Rik encodes for a DNA binding protein known as Acute Phase Response Factor (APRF) and is also referred to as Signal transducer and activator of transcription 3 (STAT3). It is associated with the acute-phase response of the immune system and is involved in regulating gene expression. APRF/STAT3 plays a key role in various biological processes, including cell growth, differentiation, apoptosis, and inflammation. The protein is encoded by the human STAT3 gene which is located on chromosome 17 and consists of 24 exons. Other names for STAT3 gene include ADMIO, APRF, AW109958, FLJ20882, HIES, and MGC16063. Understanding the function of this gene and protein is important for the development of new therapies and treatments for various diseases and conditions.

Source

Rabbit

Specificity

The detection of endogenous levels of STAT3 solely occurs when the protein is phosphorylated at Tyrosine 705, as demonstrated by the Phospho-STAT3 (Tyr705) Antibody. To create a highly similar version of the original content, we could rearrange the information as follows: The Phospho-STAT3 (Tyr705) Antibody is designed to specifically recognize STAT3 when it is phosphorylated at Tyrosine 705. It allows for the detection of endogenous levels of phosphorylated STAT3, thereby enabling the study of its role in various cellular processes.

Predictive reaction species

Pig;Rabbit;Chicken;Bovine;Horse;Zebrafish;

Application

For Western blotting, dilute at 1:500-1:2000. For immunohistochemistry, dilute at 1:50-1:200. This antibody can also be used for immunoprecipitation and immunofluorescence/immunocytochemistry at dilutions of 1:100-1:500. When used in an ELISA assay with peptide substrates, a dilution of 1:20000-1:40000 is recommended. It's important to note that proper dilution is key to achieving optimal results in any application.

STAT3 is a human protein that plays a crucial role in cell signaling pathways. Specifically, there is a site on STAT3 known as Tyrosine 705, which undergoes phosphorylation. To create a synthesized peptide, this region of human STAT3, close to the phosphorylation site, is utilized. It is important to note that the generated content should be based on the original text information and not follow the conversational approach of ChapGPT.

Properties

1mg/ml

Purification

The purification process of obtaining the antibody involves sequential chromatography on phospho- and non-phospho-peptide affinity columns using purified rabbit serum. To generate a highly similar content, the original text information can be rearranged in the following way:
The affinity-purified antibody is derived from purified rabbit serum and obtained through a series of chromatography steps. Sequential chromatography is performed on both phospho- and non-phospho-peptide affinity columns to achieve purification.

Clonality

Polyclonal Antibody

To ensure optimum preservation, it is advised to store the product at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may impact its quality.

Storage buffer

The storage condition for Rabbit IgG is freezing it at -20 °C in phosphate buffered saline with a pH value of 7.4. The solution also contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. This particular product can be stored for a period of 12 months starting from the date of receipt.

Target

Background

 Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).

Tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Celluar localization

Cytosol;Mitochondrion;Nucleus;Plasma Membrane;

P40763