Phospho-ACC1 (Ser80) Rabbit Polyclonal Antibody#abs131046

abs131046-50ug

1-2 Weeks

201

abs131046-100ug

1-2 Weeks

301

Description

ACC1 is a crucial subunit within the acetyl-CoA carboxylase (ACC) enzyme system. Its primary function is to catalyze the crucial carboxylation process that converts acetyl-CoA into malonyl-CoA. This step is considered the rate-limiting process in the synthesis of fatty acids. As such, ACC1 plays a critical role in the regulation of fatty acid metabolism and homeostasis within the body. Understanding the function and importance of ACC1 is essential to developing a comprehensive understanding of metabolic processes and their impact on overall health and wellness.

Other names

Alpha-carboxylase acetyl CoA (ACACA) and ACACA_HUMAN are known as acetyl CoA carboxylase alpha. ACACB, ACC alpha, ACC, ACC beta, ACC1, ACC2, ACCA, and ACCB are all alternative names for acetyl CoA carboxylase beta. ACACA and ACACB are enzymes that play a crucial role in fatty acid synthesis by catalyzing the carboxylation of acetyl CoA to produce malonyl CoA. These enzymes are essential for regulating lipid metabolism in the body. The genes encoding ACACA and ACACB are COA1 and COA2, respectively. The protein products of these genes are biotin carboxylase and HACC275. The other names mentioned are gene aliases or Uniprot identifiers for these enzymes. These enzymes are crucial for cellular energy homeostasis and have been implicated in various metabolic disorders.

Source

Rabbit

Specificity

ACC1 is a protein that can be detected using an antibody called Phospho-ACC1 (Ser80). This antibody specifically targets the phosphorylated form of ACC1 at Serine 80. It is important to note that the antibody is designed to detect endogenous levels of ACC1 only when it is phosphorylated at this specific site. By utilizing this antibody, researchers can gain insights about the regulation and activity of ACC1 in various biological processes.

Predictive reaction species

Pig;Sheep;Dog;Bovine;

Application

The recommended dilution factors for using this antibody in different applications are as follows: Western blotting at a dilution range of 1:500-1:2000, immunohistochemistry at a dilution range of 1:50-1:200, immunofluorescence or immunocytochemistry at a dilution range of 1:100-1:500, and ELISA (peptide) at a dilution range of 1:20000-1:40000. To obtain accurate results, it's important to use the appropriate dilution factor for each application. Therefore, it's recommended to perform a preliminary titration experiment to determine the optimal dilution factor for each specific assay. This will ensure that you get the most reliable and accurate results possible.

ACC180。，。ChapGPT，。
80ACC1。

Properties

1mg/ml

Purification

The rabbit serum was purified to obtain the antibody using a process that involved sequential chromatography on affinity columns for phospho- and non-phospho-peptides. This resulted in a highly specific antibody that can recognize its target with high affinity. The affinity purification technique used in this process ensures that the antibody is of high quality and purity, making it suitable for various applications in biological and medical research. Overall, the purified rabbit serum-derived antibody has great potential as an important tool in the field of life sciences.

Clonality

Polyclonal Antibody

It is recommended to keep the product in a freezer at a temperature of -20 °C for up to a year. To prevent any damage to the product, avoid exposing it to repeated freeze/thaw cycles.

Storage buffer

The Rabbit IgG is stored in a solution of phosphate buffered saline at a pH of 7.4, with 150mM NaCl and 0.02% sodium azide, adding in 50% glycerol for stability. It should be stored at a temperature of -20°C and can remain stable for up to 12 months from the date of receipt.

Target

Background

The enzyme involved in the crucial step of long-chain fatty acid production is known as the rate-limiting reaction catalyst. It performs three essential functions which are the biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase. By rearranging the given information, the following highly similar content is generated: The rate-limiting reaction in the biogenesis of long-chain fatty acids is catalyzed by an enzyme that carries out three important functions - biotin carboxyl carrier protein, biotin carboxylase, and carboxyltransferase.

Tissue specificity

Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus;

Q13085