Recombinant Rat TNF-α/TNFSF2 #abs01068

abs01068-5ug

1-2 Weeks

138

abs01068-20ug

1-2 Weeks

344

abs01068-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and subsequently lyophilized. The original solution was prepared in 20 mM PB with a pH of 7.2 and contained 150 mM NaCl. A highly similar content can be generated from this information by rearranging it in various ways.

Properties

aa_sequence

FGVIAL SDQLVNPEAT KGDLLESAVY LDITESGQPV YFGVQVLFKG CPDYVLLTH TCCPKDTPEG AELKPWYEP MYLGGVFQL EARFAISYQE KVSLLSAIKS PCQRANALLA NGMDLKDNQL VVPADGLYLI YSQVLFKGQ GCPDYVLLTH TVSRFAISYQ EKVQRANALL ANGMDLKDNQ LVVPADGLYL IYSQVLFKGQ GCPDYVLLTH TVSRFAISYQ EKVLRSSSQNS SDKPVAHVVN HQAEEQLEWL SPKFVANRGQ M.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has shown that the amount of rRtTNF-α is less than 1 EU/μg. This information indicates that the product is of high quality and meets the required standards.

Activity

The potency of the sample is equivalent to that of the standard, exhibiting complete biological activity. The effective dose remains unchanged, demonstrating that the sample can be utilized as a substitute for the standard.₅₀The specific activity of the substance, which was measured by a cytotoxicity assay using murine L929 cells, is greater than 2.0 × 10, with a corresponding ng/ml value of less than 0.05. This demonstrates the highly potent nature of the substance and its ability to induce cell death at very low concentrations. It is important to understand the potency of such substances in order to establish safe levels of exposure and avoid any potential harmful effects on human health.⁷ IU/mg in the presence of actinomycin D.

Reconstitution

To ensure proper mixing of the contents, we suggest briefly centrifuging this vial before opening. To reconstitute the substance, utilize sterile distilled water or an aqueous buffer that contains 0.1% BSA, until reaching a concentration of 0.1-1.0 mg/mL. Stock solutions should be separated into smaller working aliquots and stored at a temperature less than or equal to -20°C. For further dilution, employ appropriate buffered solutions.

Stability & Storage

To ensure the stability of this lyophilized preparation, it should be stored at a temperature range of 2-8 °C. However, for long-term storage, it is advisable to keep it at -20 °C after desiccation. Upon reconstitution, the preparation remains stable for up to one week at a temperature range of 2-8 °C. For enhanced stability, it is recommended that you distribute the reconstituted preparation into smaller working aliquots and store them at temperatures between -20 °C and -70 °C. Avoid exposing the preparation to frequent freeze/thaw cycles as this can lead to instability and ineffectiveness.

Target

Accession

P16599

Gene IDs

24835

References

References

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.

2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.

3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.

4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.