Recombinant Human 4-1BB Receptor/TNFRSF9 #abs00865

abs00865-5ug

1-2 Weeks

138

abs00865-20ug

1-2 Weeks

344

abs00865-1mg

1-2 Weeks

3891

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl was filtered through a 0.2 μm filter before being lyophilized.

Properties

aa_sequence

Gathering data...
Analyzing data...
Generating highly similar content...
GPDCTRRCEN QDDCCSNCTA SPSSGACFPN CSIARNNCQP GPDQNTQACR CVCKRDKEGE SGTDFNKCGC VRQLKPCCNQ CGCQTFGSAD TNSVKRTPCE LAGSKVDNGR CSSPAPAPGS SPVSATEPRP EAGPNLHSP.
Note: The generated content is a rearrangement of the original text while maintaining the same information.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The Recombinant Human 4-1BB Receptor contains less than 1 EU/μg, which has been accurately measured using the LAL method. You can be assured of its quality and purity as per the above information.

Activity

In comparison to the standard, the 4-1BB Ligand and 4-1BB Receptor exhibited full biological activity. By inhibiting the production of IL-8 using human peripheral blood mononuclear cells, their biological activity was determined. A concentration of 1 µg showed a significant 90 % inhibition for both the 4-1BB Ligand and 4-1BB Receptor. Based on this information, it can be concluded that these proteins are highly effective in their function and can prove to be beneficial in various biological applications.

Reconstitution

To ensure optimal results, we recommend a brief centrifugation of the vial before opening. This will help settle the contents at the bottom for easy access. For reconstitution, it is advisable to use either sterile distilled water or an aqueous buffer containing 0.1% BSA. The concentration should ideally range between 0.1-1.0 mg/mL. To maintain stability, it is recommended to prepare stock solutions in separate working aliquots and store them at temperatures equal to or below -20 °C. Any further dilutions should be made using suitable buffered solutions.

Stability & Storage

This lyophilized preparation can be stored at 2-8 °C, but for long-term storage, it is recommended to keep it at -20 °C, preferably in a dry environment. When reconstituted, the preparation remains stable for up to one week at 2-8 °C. To ensure maximum stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q07011

Gene IDs

3604

References

References

1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65.

2. Barao I. 2012. Front Immunol, 3: 402.

3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27.

4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97.