Recombinant Human TNF-α/TNFSF2 #abs00847

abs00847-10ug

In Stock

138

abs00847-50ug

In Stock

344

abs00847-1mg

In Stock

1945

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, 10 mM Nacl, pH 7.0 was filtered through a 0.2 μm filter and then lyophilized. To create similar content based on this information, one could write: The solution was first concentrated and then filtered using a 0.2 μm filter before undergoing lyophilization. The original solution contained 20 mM PB, 10 mM Nacl, and had a pH of 7.0.

Properties

aa_sequence

RSYTQSAVLI LYYILGESPQ VLQWLGQEAP ANLALNARRN LQGEAQGEPV NVAHVAPKPD SSRTRSSVVM LYHVSTLHLP IILLLTHVHP QASYYAVRIS KSKIAVSLLN ETTGEPQCRP YWIYPEKPAE VLQKGQFVGK IAEFDNNRAS LQGGIYFVYF.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of Recombinant Human TNF-α/TNFSF2 was determined to be below 1.0 EU/μg using the LAL method. Please provide a rephrased response based on the original text information.

Activity

Compared to the standard, it has complete biological activity. The effectiveness is equivalent to the original.₅₀The specific activity of less than 0.05 ng/ml, as determined by a cytotoxicity assay using murine L929 cells, corresponds to a highly potent effect of > 2.0 × 10.⁷ IU/mg in the presence of actinomycin D.

Reconstitution

To ensure that the contents settle at the bottom, we suggest briefly centrifuging this vial before opening. Reconstitute the contents in sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide stock solutions into working aliquots and store them at or below -20 °C. For further dilutions, use appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at a temperature of 2-8 °C, although long-term storage should be done at -20 °C and in a desiccated state. Upon reconstitution, you can keep the preparation stable for up to a week at a temperature of 2-8 °C. However, for maximum stability, it is advisable to divide the reconstituted preparation into working aliquots and then store them within a temperature range of -20 °C to -70 °C. It is important to avoid repeated freeze/thaw cycles, which can compromise the quality of the preparation.

Target

Accession

P01375

Gene IDs

7124

References

References

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.

2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.

3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.

4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.